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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-1-9
|
| pubmed:abstractText |
Studies of the regulation of the IgE synthesis have been hampered by the difficulties in making lymphocytes produce IgE in vitro. Synthesis of IgE, IgG1 and IgM were investigated in a murine coculture system in which a conalbumin-specific T helper cell line of Th2 type was cultured together with nonadherent splenocytes from normal MHC-matched mice. Help provided by the antigen-stimulated T cell line induced significant IgE production (20 ng/ml), along with IgG1 (5 micrograms/ml) and IgM (250 micrograms/ml). Immunoglobulin synthesis in cultures was detectable at day 3-4 and culminated at day 7-8. IL-4 production was observed within the first 2 days of culture. The kinetics of the synthesis of the three isotypes were parallel. Stimulation of the Th2 cell line with Con A produced dose-response curves resembling those after antigen stimulation, whereas supernatant from these cells was unable to induce synthesis of IgE. The presence of interferon-gamma in the cultures, inhibited synthesis of IgE, IgM and IgG1, but this inhibition was not due to interference with the IL-4 production, which increased in the presence of high doses of interferon-gamma.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Conalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0020-5915
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
95
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
363-8
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1835713-Animals,
pubmed-meshheading:1835713-Cells, Cultured,
pubmed-meshheading:1835713-Conalbumin,
pubmed-meshheading:1835713-Concanavalin A,
pubmed-meshheading:1835713-Dose-Response Relationship, Drug,
pubmed-meshheading:1835713-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1835713-Female,
pubmed-meshheading:1835713-Gene Expression Regulation,
pubmed-meshheading:1835713-Immunoglobulin E,
pubmed-meshheading:1835713-Immunoglobulin G,
pubmed-meshheading:1835713-Immunoglobulin M,
pubmed-meshheading:1835713-Interferon-gamma,
pubmed-meshheading:1835713-Interleukin-4,
pubmed-meshheading:1835713-Mice,
pubmed-meshheading:1835713-Mice, Inbred AKR,
pubmed-meshheading:1835713-Mice, Inbred C3H,
pubmed-meshheading:1835713-Radioimmunoassay,
pubmed-meshheading:1835713-T-Lymphocytes, Helper-Inducer
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
IL-4-producing murine T helper cell line provides help for in vitro production of IgE.
|
| pubmed:affiliation |
Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|