Linked Life Data

18356280

Source:http://linkedlifedata.com/resource/pubmed/id/18356280

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-6-30
pubmed:abstractText
The antiapoptotic molecule Fas-associated death domain-like IL-1beta-converting enzyme inhibitory protein (FLIP) inhibits Fas-mediated apoptosis by blocking activation of caspase-8. We previously showed that expression of transgenic FLIP on thyroid epithelial cells (TECs) of DBA/1 and CBA/J mice promoted earlier resolution of granulomatous experimental autoimmune thyroiditis in vivo. This study was undertaken to directly determine whether transgenic FLIP expressed on cultured TECs can protect TECs from Fas-mediated apoptosis in vitro. The results indicate that cultured TECs from DBA/1 and CBA/J mice can be sensitized in vitro by interferon-gamma and TNF-alpha to undergo Fas-mediated apoptosis. Transgenic overexpression of FLIP protected cultured TECs of FLIP transgene (Tg)+ DBA/1 and CBA/J mice from Fas-mediated apoptosis, and FLIP small interfering RNA transfection of cultured TECs of FLIP Tg+ DBA/1 and CBA/J mice abolished the protective effect. These in vitro results are consistent with our previous in vivo studies using DBA/1 and CBA/J FLIP Tg+ mice and provide direct support for the hypothesis that transgenic expression of FLIP promotes resolution of granulomatous experimental autoimmune thyroiditis by protecting TECs from apoptosis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-10198242, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-10209037, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-10464273, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-10642601, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-10657610, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11099302, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11101869, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11164911, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11714840, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11905814, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-11913070, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-12040174, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-14499245, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-15563545, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-16487007, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-16498450, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-17322373, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-17823262, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-7502042, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-7518929, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-7878464, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-8754734, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9020075, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9027314, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9206994, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9212099, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9254659, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9393856, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9501089, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9794838, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9841933, http://linkedlifedata.com/resource/pubmed/commentcorrection/18356280-9886394
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3321-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:18356280-Animals, pubmed-meshheading:18356280-Antigens, CD95, pubmed-meshheading:18356280-Apoptosis, pubmed-meshheading:18356280-CASP8 and FADD-Like Apoptosis Regulating Protein, pubmed-meshheading:18356280-Caspase 3, pubmed-meshheading:18356280-Caspase 8, pubmed-meshheading:18356280-Cells, Cultured, pubmed-meshheading:18356280-Epithelial Cells, pubmed-meshheading:18356280-Fas Ligand Protein, pubmed-meshheading:18356280-Immunohistochemistry, pubmed-meshheading:18356280-Interferon-gamma, pubmed-meshheading:18356280-Mice, pubmed-meshheading:18356280-Mice, Inbred CBA, pubmed-meshheading:18356280-Mice, Inbred DBA, pubmed-meshheading:18356280-Mice, Transgenic, pubmed-meshheading:18356280-Microscopy, Confocal, pubmed-meshheading:18356280-RNA, Small Interfering, pubmed-meshheading:18356280-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18356280-Thyroid Gland, pubmed-meshheading:18356280-Tumor Necrosis Factor-alpha
pubmed:year
2008
pubmed:articleTitle
Cultured murine thyroid epithelial cells expressing transgenic Fas-associated death domain-like interleukin-1beta converting enzyme inhibitory protein are protected from fas-mediated apoptosis.
pubmed:affiliation
Division of Immunology and Rheumatology, Department of Medicine, University of Missouri, NE307 Medical Sciences, Columbia, MO 65212, USA. fangy@health.missouri.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural