Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-3-20
pubmed:abstractText
High fidelity polymerases are efficient catalysts of phosphodiester bond formation during DNA replication or repair. We interpret molecular dynamics simulations of a polymerase bound to its substrate DNA and incoming nucleotide using a quasiharmonic model to study the effect of external forces applied to the bound DNA on the kinetics of phosphoryl transfer. The origin of the force dependence is shown to be an intriguing coupling between slow, delocalized polymerase-DNA modes and fast catalytic site motions. Using noncognate DNA substrates we show that the force dependence is context specific.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-10716452, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-11050232, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-12540915, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-12637673, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-12649320, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-15069184, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-15345530, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-15533035, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-15863620, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-16222654, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-16516229, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-16756501, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-17176036, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-1989886, http://linkedlifedata.com/resource/pubmed/commentcorrection/18352668-8906882
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0031-9007
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
088102
pubmed:dateRevised
2011-5-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Computational study of the force dependence of phosphoryl transfer during DNA synthesis by a high fidelity polymerase.
pubmed:affiliation
Department of Bioengineering , University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.