Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-4-16
pubmed:abstractText
Mitochondria play essential roles in cardiac pathophysiology and the murine model has been extensively used to investigate cardiovascular diseases. In the present study, we characterized murine cardiac mitochondria using an LC/MS/MS approach. We extracted and purified cardiac mitochondria; validated their functionality to ensure the final preparation contains necessary components to sustain their normal function; and subjected these validated organelles to LC/MS/MS-based protein identification. A total of 940 distinct proteins were identified from murine cardiac mitochondria, among which, 480 proteins were not previously identified by major proteomic profiling studies. The 940 proteins consist of functional clusters known to support oxidative phosphorylation, metabolism, and biogenesis. In addition, there are several other clusters, including proteolysis, protein folding, and reduction/oxidation signaling, which ostensibly represent previously under-appreciated tasks of cardiac mitochondria. Moreover, many identified proteins were found to occupy other subcellular locations, including cytoplasm, ER, and golgi, in addition to their presence in the mitochondria. These results provide a comprehensive picture of the murine cardiac mitochondrial proteome and underscore tissue- and species-specification. Moreover, the use of functionally intact mitochondria insures that the proteomic observations in this organelle are relevant to its normal biology and facilitates decoding the interplay between mitochondria and other organelles.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-10891285, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-11041865, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-11080252, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-11444914, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12038767, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12169683, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12573857, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12592411, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12663490, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12835951, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12865426, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-12933700, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-14576278, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-14651853, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-14681408, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-15608177, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-15608235, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-15608251, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-15707357, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16093501, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16231421, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16381842, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16381964, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16415296, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16489745, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16582907, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16615898, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16615899, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16733263, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16823961, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16876102, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-16912974, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-17000643, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-17137336, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-3663110, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-5650364, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-7137598, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-7379260, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-9399864, http://linkedlifedata.com/resource/pubmed/commentcorrection/18348319-9638947
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1615-9861
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1564-75
pubmed:dateRevised
2011-4-21
pubmed:meshHeading
pubmed:year
2008
More...