| pubmed-article:1834549 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0024501 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0022688 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0108788 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0108789 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0439590 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C1517945 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1834549 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1834549 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1834549 | pubmed:dateCreated | 1991-12-6 | lld:pubmed |
| pubmed-article:1834549 | pubmed:abstractText | The results of the present study show that activation-induced changes in CD45RA and CD45RO expression on T cells and natural killer (NK) cells are not unidirectional for all cells during a 5-week culture period. T cells and NK cells were generated from a resting subpopulation of peripheral blood mononuclear cells (PBMC) defined by sedimentation at Percoll high buoyant densities (p greater than 1.0640 g/ml) and unresponsiveness to IL-2. T cells were activated by a combination of PHA, sheep erythrocytes and IL-2-conditioned medium (IL-2-CM), and NK cells were activated by co-culture with gamma-irradiated malignant melanoma (MM-170) cells and IL-2-CM. Both T-cell and NK-cell cultures were maintained by subculture in IL-2-CM. NK cells and the CD45R(Abright)RO(dim/neg) subpopulation of T cells gained CD45RO following activation and this was accompanied by a two-fold decrease in CD45RA expression. In different cultures, CD45RO expression was not stable on 28-80% of T cells and 10-55% of NK cells. Cells with decreased CD45RO expression showed increased expression of CD45RA. Instability of CD45RO expression on cultured T cells and NK cells occurred at a time following the period of rapid cell growth when the cells were entering a quiescent phase. Both the CD4+ and CD8+ T-cell subpopulation showed similar changes in CD45 isoform expression. In contrast to the results obtained with the CD45R(Abright)RO(dim/neg) resting T cells, the CD45RO(bright)RA(dim/neg) subpopulation of resting T cells when activated and cultured under identical conditions retained CD45RO expression and remained CD45RAdim/neg. Thus a significant proportion of resting CD45R(Abright)RO(dim/neg) T cells is not related in a differentiation sequence to resting CD45RObrightRAdim/neg T cells, and therefore resting CD45RAbrightROdim/neg T cells and resting NK cells may be heterogeneous with respect to their activation history. | lld:pubmed |
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| pubmed-article:1834549 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1834549 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1834549 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1834549 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1834549 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1834549 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:1834549 | pubmed:author | pubmed-author:WarnerD WDW | lld:pubmed |
| pubmed-article:1834549 | pubmed:author | pubmed-author:SkipseyL JLJ | lld:pubmed |
| pubmed-article:1834549 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1834549 | pubmed:volume | 74 | lld:pubmed |
| pubmed-article:1834549 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1834549 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1834549 | pubmed:pagination | 78-85 | lld:pubmed |
| pubmed-article:1834549 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1834549 | pubmed:meshHeading | pubmed-meshheading:1834549-... | lld:pubmed |
| pubmed-article:1834549 | pubmed:meshHeading | pubmed-meshheading:1834549-... | lld:pubmed |
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| pubmed-article:1834549 | pubmed:meshHeading | pubmed-meshheading:1834549-... | lld:pubmed |
| pubmed-article:1834549 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1834549 | pubmed:articleTitle | Loss of activation-induced CD45RO with maintenance of CD45RA expression during prolonged culture of T cells and NK cells. | lld:pubmed |
| pubmed-article:1834549 | pubmed:affiliation | Cancer Research Unit, Royal Canberra Hospital South, Woden, Australian Capital Territory. | lld:pubmed |
| pubmed-article:1834549 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1834549 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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