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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1991-12-6
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pubmed:abstractText |
The results of the present study show that activation-induced changes in CD45RA and CD45RO expression on T cells and natural killer (NK) cells are not unidirectional for all cells during a 5-week culture period. T cells and NK cells were generated from a resting subpopulation of peripheral blood mononuclear cells (PBMC) defined by sedimentation at Percoll high buoyant densities (p greater than 1.0640 g/ml) and unresponsiveness to IL-2. T cells were activated by a combination of PHA, sheep erythrocytes and IL-2-conditioned medium (IL-2-CM), and NK cells were activated by co-culture with gamma-irradiated malignant melanoma (MM-170) cells and IL-2-CM. Both T-cell and NK-cell cultures were maintained by subculture in IL-2-CM. NK cells and the CD45R(Abright)RO(dim/neg) subpopulation of T cells gained CD45RO following activation and this was accompanied by a two-fold decrease in CD45RA expression. In different cultures, CD45RO expression was not stable on 28-80% of T cells and 10-55% of NK cells. Cells with decreased CD45RO expression showed increased expression of CD45RA. Instability of CD45RO expression on cultured T cells and NK cells occurred at a time following the period of rapid cell growth when the cells were entering a quiescent phase. Both the CD4+ and CD8+ T-cell subpopulation showed similar changes in CD45 isoform expression. In contrast to the results obtained with the CD45R(Abright)RO(dim/neg) resting T cells, the CD45RO(bright)RA(dim/neg) subpopulation of resting T cells when activated and cultured under identical conditions retained CD45RO expression and remained CD45RAdim/neg. Thus a significant proportion of resting CD45R(Abright)RO(dim/neg) T cells is not related in a differentiation sequence to resting CD45RObrightRAdim/neg T cells, and therefore resting CD45RAbrightROdim/neg T cells and resting NK cells may be heterogeneous with respect to their activation history.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1671403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1692083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1696528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1825480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1968889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1974177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1978253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2138599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2144907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2466769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2473122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2525111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2530273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2531196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2894392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2965180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2974420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2978372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-3487503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-3496271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-6352802
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-2805
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
78-85
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1834549-Antigens, CD,
pubmed-meshheading:1834549-Antigens, CD45,
pubmed-meshheading:1834549-Cells, Cultured,
pubmed-meshheading:1834549-Histocompatibility Antigens,
pubmed-meshheading:1834549-Humans,
pubmed-meshheading:1834549-Killer Cells, Natural,
pubmed-meshheading:1834549-Lymphocyte Activation,
pubmed-meshheading:1834549-T-Lymphocytes,
pubmed-meshheading:1834549-Time Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Loss of activation-induced CD45RO with maintenance of CD45RA expression during prolonged culture of T cells and NK cells.
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pubmed:affiliation |
Cancer Research Unit, Royal Canberra Hospital South, Woden, Australian Capital Territory.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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