Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-12-6
pubmed:abstractText
The results of the present study show that activation-induced changes in CD45RA and CD45RO expression on T cells and natural killer (NK) cells are not unidirectional for all cells during a 5-week culture period. T cells and NK cells were generated from a resting subpopulation of peripheral blood mononuclear cells (PBMC) defined by sedimentation at Percoll high buoyant densities (p greater than 1.0640 g/ml) and unresponsiveness to IL-2. T cells were activated by a combination of PHA, sheep erythrocytes and IL-2-conditioned medium (IL-2-CM), and NK cells were activated by co-culture with gamma-irradiated malignant melanoma (MM-170) cells and IL-2-CM. Both T-cell and NK-cell cultures were maintained by subculture in IL-2-CM. NK cells and the CD45R(Abright)RO(dim/neg) subpopulation of T cells gained CD45RO following activation and this was accompanied by a two-fold decrease in CD45RA expression. In different cultures, CD45RO expression was not stable on 28-80% of T cells and 10-55% of NK cells. Cells with decreased CD45RO expression showed increased expression of CD45RA. Instability of CD45RO expression on cultured T cells and NK cells occurred at a time following the period of rapid cell growth when the cells were entering a quiescent phase. Both the CD4+ and CD8+ T-cell subpopulation showed similar changes in CD45 isoform expression. In contrast to the results obtained with the CD45R(Abright)RO(dim/neg) resting T cells, the CD45RO(bright)RA(dim/neg) subpopulation of resting T cells when activated and cultured under identical conditions retained CD45RO expression and remained CD45RAdim/neg. Thus a significant proportion of resting CD45R(Abright)RO(dim/neg) T cells is not related in a differentiation sequence to resting CD45RObrightRAdim/neg T cells, and therefore resting CD45RAbrightROdim/neg T cells and resting NK cells may be heterogeneous with respect to their activation history.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1671403, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1692083, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1696528, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1825480, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1968889, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1974177, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-1978253, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2138599, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2144907, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2466769, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2473122, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2525111, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2530273, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2531196, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2894392, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2965180, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2974420, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-2978372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-3487503, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-3496271, http://linkedlifedata.com/resource/pubmed/commentcorrection/1834549-6352802
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0019-2805
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
78-85
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Loss of activation-induced CD45RO with maintenance of CD45RA expression during prolonged culture of T cells and NK cells.
pubmed:affiliation
Cancer Research Unit, Royal Canberra Hospital South, Woden, Australian Capital Territory.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't