|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2008-4-11
|
|
pubmed:abstractText |
Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y(1,12&13) receptors. ADP (10microM) evoked increases in intracellular calcium that were essentially abolished by the P2Y(1) receptor antagonist MRS2179 (10microM), responses were also absent in preparations from P2Y(1) receptor deficient mice however UTP (100microM) evoked calcium rises were unaffected. ADP also evoked a robust increase in extracellular signal-regulated protein kinase (ERK) phosphorylation that was of similar magnitude in the cultures from wild type and P2Y(1) receptor deficient mice. These results suggest that ADP acts through P2Y(1) receptors to mediate an increase in intracellular calcium but not to stimulate ERK phosphorylation in the spinal cord.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-10606627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-10751431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-11278310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-12540525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-12657694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-12805284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-15339647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-15466449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-15728851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-16231090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-16257449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18343032-7858877
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Aniline Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fluo-3,
http://linkedlifedata.com/resource/pubmed/chemical/N(6)-methyl-2'-deoxyadenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/P2ry1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P2 Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2Y1,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthenes
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0304-3940
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
25
|
|
pubmed:volume |
435
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
190-3
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18343032-Adenosine Diphosphate,
pubmed-meshheading:18343032-Aniline Compounds,
pubmed-meshheading:18343032-Animals,
pubmed-meshheading:18343032-Calcium,
pubmed-meshheading:18343032-Dose-Response Relationship, Drug,
pubmed-meshheading:18343032-Drug Interactions,
pubmed-meshheading:18343032-Female,
pubmed-meshheading:18343032-Male,
pubmed-meshheading:18343032-Mice,
pubmed-meshheading:18343032-Mice, Inbred C57BL,
pubmed-meshheading:18343032-Mice, Knockout,
pubmed-meshheading:18343032-Purinergic P2 Receptor Antagonists,
pubmed-meshheading:18343032-Receptors, Purinergic P2,
pubmed-meshheading:18343032-Receptors, Purinergic P2Y1,
pubmed-meshheading:18343032-Signal Transduction,
pubmed-meshheading:18343032-Spinal Cord,
pubmed-meshheading:18343032-Time Factors,
pubmed-meshheading:18343032-Tissue Culture Techniques,
pubmed-meshheading:18343032-Uridine Triphosphate,
pubmed-meshheading:18343032-Xanthenes
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Contribution of P2Y(1) receptors to ADP signalling in mouse spinal cord cultures.
|
|
pubmed:affiliation |
Department of Cell Physiology & Pharmacology, Henry Wellcome Building, University of Leicester, Leicester LE1 9HN, UK.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
