|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2008-4-14
|
|
pubmed:abstractText |
Interferon-alpha (IFN-alpha) has a critical role in antiviral immunity and plasmacytoid dendritic cells (pDCs) have been demonstrated as the principal IFN-alpha source after Toll-like receptor (TLR) 7 and 9 stimulation. Little is known about the contribution of pDC-independent IFN-alpha sources to total IFN-alpha production capacity of human peripheral blood. Using an array of pathogen associated molecular patterns (PAMPs), Poly(I:C)/Dotap represented the second strongest IFN-alpha stimulus in total PBMC. Poly(I:C)/Dotap induced three times more IFN-alpha, when compared to TLR7-stimulation (R848) and four times less, when compared to TLR9-stimulation. Dotap (mediator of cellular uptake) dramatically increased Poly(I:C)-induced IFN-alpha production. Sorting experiments and ELISpot assays revealed that monocytes and not myeloid DCs are the main IFN-alpha source after Poly(I:C)/Dotap stimulation. ELISpot analyses demonstrated the highest IFN-alpha spot numbers after Poly(I:C)/Dotap stimulation. Although pDCs produced highest IFN-alpha levels per cell, monocytes represent a competing IFN-alpha source in total PBMC due to their high frequency.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-dioleoyloxy-3-(trimethylammonium...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/CpG ODN 2006,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Monounsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Poly I-C,
http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/R 848,
http://linkedlifedata.com/resource/pubmed/chemical/TLR7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 7
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
1521-7035
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
127
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
252-64
|
|
pubmed:meshHeading |
pubmed-meshheading:18342575-Adult,
pubmed-meshheading:18342575-Antigens, CD14,
pubmed-meshheading:18342575-Fatty Acids, Monounsaturated,
pubmed-meshheading:18342575-Flow Cytometry,
pubmed-meshheading:18342575-Fluorescent Dyes,
pubmed-meshheading:18342575-Humans,
pubmed-meshheading:18342575-Imidazoles,
pubmed-meshheading:18342575-Immunophenotyping,
pubmed-meshheading:18342575-Interferon-alpha,
pubmed-meshheading:18342575-Male,
pubmed-meshheading:18342575-Monocytes,
pubmed-meshheading:18342575-Oligodeoxyribonucleotides,
pubmed-meshheading:18342575-Poly I-C,
pubmed-meshheading:18342575-Quaternary Ammonium Compounds,
pubmed-meshheading:18342575-Statistics, Nonparametric,
pubmed-meshheading:18342575-Toll-Like Receptor 7
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Human monocytes represent a competitive source of interferon-alpha in peripheral blood.
|
|
pubmed:affiliation |
Institute for Clinical Immunology and Transfusion Medicine, Justus-Liebig-University Giessen, 35385 Giessen, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
