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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1991-11-15
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pubmed:abstractText |
We have developed a procedure for the affinity purification of small nuclear ribonucleoproteins (snRNPs) of Trypanosoma brucei (U2 and U4/U6 snRNPs), which are essential for trans splicing. Each of these snRNPs can be specifically and efficiently selected from T. brucei extracts through biotinylated antisense 2'-O-methylated RNA oligonucleotides immobilized on streptavidin-agarose. Protein analysis revealed a set of five low molecular weight polypeptides common to the U2 and U4/U6 snRNPs and the spliced leader RNP. In addition, several U2 and U4/U6 snRNP-specific protein components were identified. Using monoclonal antibodies against human snRNP proteins, we could not detect any significant cross-reaction with the trypanosomal U2 snRNP proteins. Thus, the trypanosomal snRNPs exhibit principal differences from the higher eukaryotic snRNPs not only in their RNA but also in their protein components.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-1692263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-1701240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-1826044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2141334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2142018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2168293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2477448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2531659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2582042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2675423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2725495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2726482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2951249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2971142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-2977088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-3060859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-3120186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-3327783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-3419533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-3786140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-43092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-6091897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833773-6789322
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9097-101
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1833773-Animals,
pubmed-meshheading:1833773-Antibodies, Monoclonal,
pubmed-meshheading:1833773-Base Sequence,
pubmed-meshheading:1833773-Cross Reactions,
pubmed-meshheading:1833773-Humans,
pubmed-meshheading:1833773-Immunoblotting,
pubmed-meshheading:1833773-Molecular Sequence Data,
pubmed-meshheading:1833773-Nucleic Acid Conformation,
pubmed-meshheading:1833773-Oligonucleotides, Antisense,
pubmed-meshheading:1833773-RNA, Small Nuclear,
pubmed-meshheading:1833773-RNA Splicing,
pubmed-meshheading:1833773-Ribonucleoproteins,
pubmed-meshheading:1833773-Ribonucleoproteins, Small Nuclear,
pubmed-meshheading:1833773-Trypanosoma brucei brucei
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pubmed:year |
1991
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pubmed:articleTitle |
Affinity purification of Trypanosoma brucei small nuclear ribonucleoproteins reveals common and specific protein components.
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pubmed:affiliation |
Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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