Linked Life Data

18337694

Source:http://linkedlifedata.com/resource/pubmed/id/18337694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-3-13
pubmed:abstractText
The sigmas subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1225-8873
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
56-61
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
5' Untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation.
pubmed:affiliation
Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444/a, PO Box 23, 11010 Belgrade, Serbia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't