Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
2008-4-14
pubmed:abstractText
Catalpol, an iridoid glycoside abundant in the roots of Rehmannia glutinosa, has been previously found to prevent the loss of CA1 hippocampal neurons and to reduce working errors in gerbils after ischemia-reperfusion injury. In the present study, we investigated the effects of catalpol on astrocytes in an ischemic model to further characterize its neuroprotective mechanisms. Primary cultured astrocytes exposed to oxygen-glucose deprivation (OGD) followed by reperfusion (adding back oxygen and glucose, OGD-R), were used as an in vitro ischemic model. Treatment of the astrocytes with catalpol during ischemia-reperfusion increased astrocyte survival significantly in a concentration-dependent manner, as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) release and morphological observation. In addition, catalpol prevented the decrease in mitochondrial membrane potential, inhibited the formation of reactive oxygen species (ROS) and the production of nitric oxide (NO), decreased the level of lipid peroxide and the activity of inducible nitric oxide synthase (iNOS), and elevated the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and the content of glutathione (GSH). Our results suggest that catalpol exerts the most significant cytoprotective effect on astrocytes by suppressing the production of free radicals and elevating antioxidant capacity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants, http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase, http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents, http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds, http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species, http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase, http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts, http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles, http://linkedlifedata.com/resource/pubmed/chemical/catamine AB, http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
pubmed:status
MEDLINE
pubmed:issn
0736-5748
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-17
pubmed:meshHeading
pubmed-meshheading:18337048-Animals, pubmed-meshheading:18337048-Antioxidants, pubmed-meshheading:18337048-Astrocytes, pubmed-meshheading:18337048-Brain Ischemia, pubmed-meshheading:18337048-Cell Survival, pubmed-meshheading:18337048-Cells, Cultured, pubmed-meshheading:18337048-Dose-Response Relationship, Drug, pubmed-meshheading:18337048-Free Radicals, pubmed-meshheading:18337048-Glutathione, pubmed-meshheading:18337048-Glutathione Peroxidase, pubmed-meshheading:18337048-L-Lactate Dehydrogenase, pubmed-meshheading:18337048-Lipid Peroxidation, pubmed-meshheading:18337048-Membrane Potential, Mitochondrial, pubmed-meshheading:18337048-Mice, pubmed-meshheading:18337048-Models, Biological, pubmed-meshheading:18337048-Neuroprotective Agents, pubmed-meshheading:18337048-Oxidative Stress, pubmed-meshheading:18337048-Quaternary Ammonium Compounds, pubmed-meshheading:18337048-Reactive Oxygen Species, pubmed-meshheading:18337048-Reperfusion Injury, pubmed-meshheading:18337048-Superoxide Dismutase, pubmed-meshheading:18337048-Tetrazolium Salts, pubmed-meshheading:18337048-Thiazoles
pubmed:articleTitle
Catalpol protects primary cultured astrocytes from in vitro ischemia-induced damage.
pubmed:affiliation
School of Environmental and Biological Science & Technology, Dalian University of Technology, Dalian, Liaoning, China.
pubmed:publicationType
Journal Article