Linked Life Data

18336353

Source:http://linkedlifedata.com/resource/pubmed/id/18336353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-3-13
pubmed:abstractText
Intestinal trefoil factor (ITF), a member of the trefoil factor family, plays an important role in protecting the epithelial layer of the intestinal tract from damage and repairing epithelium after injury. In the present study, we expressed recombinant hITF by Picha pastoris expression system in shake flasks to 50mg/L. rhITF was purified up to 95% by ammonium sulfate precipitation, Ni-NTA affinity chromatography and ultrafiltration. In conditions simulating intestinal tract environment, rhITF was shown to exhibit resistance to proteases, heat, acid and alkali. In addition, rhITF was found to be biologically active in an in vitro restitution model. This study lays the foundation for the commercial production of rhITF and provides theoretical evidence for the oral use of rhITF.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0929-8665
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Stability and biological activity of human intestinal trefoil factor produced by Pichia pastoris.
pubmed:affiliation
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing 400038, P.R. China. pxlrmm@163.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't