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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0016030,
umls-concept:C0021755,
umls-concept:C0026809,
umls-concept:C0028778,
umls-concept:C0028953,
umls-concept:C0035696,
umls-concept:C0063710,
umls-concept:C0086418,
umls-concept:C0591833,
umls-concept:C1280500
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pubmed:issue |
4
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pubmed:dateCreated |
1991-11-1
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pubmed:abstractText |
Phosphodiester and phosphorothioate oligodeoxynucleotides (18 mers) were constructed antisense to sequences of the recently cloned murine and human IL-1 receptors. Murine antisense oligonucleotides inhibited IL-1-stimulated PGE2 synthesis by murine fibroblasts in culture in a time (days) and concentration-dependent (3 microM-30 microM) fashion. Murine sense oligonucleotide and an oligonucleotide antisense to human IL-1 receptor were without effect. Moreover, murine antisense oligonucleotides did not affect tumor necrosis factor- or bradykinin-stimulated PGE2 synthesis by murine fibroblasts. Similarly, antisense oligonucleotides to the human, but not the murine, IL-1 receptor inhibited IL-1-stimulated PGE2 synthesis by cultured human fibroblasts. The attenuation of the cellular response to IL-1 caused by the antisense oligonucleotides correlated with a loss in cell surface receptors for IL-1, without any change in the number of bradykinin receptors on these cells. When antisense oligonucleotides were encapsulated in liposomes, they blocked completely the appearance of newly synthesized IL-1 receptors and IL-1-stimulated PGE2 synthesis. In mice, subcutaneous injection with an oligonucleotide antisense to the murine IL-1 receptor markedly inhibited the infiltration of neutrophils in response to subsequent injection of IL-1. These data suggest that antisense oligodeoxynucleotides may share a role in the design of antiinflammatory therapeutics.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-1704400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-1850511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2125233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2126672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2137201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2139180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2448799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2466396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2477442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2483657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2508441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2521831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2524527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2524832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2530579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2530580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2530587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2532321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2649287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2726730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2836790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2839827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2850056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2901097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2945861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2952728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2958006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2969618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2969785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-2971671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-3056098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-3063445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-3148560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-6254391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-6423734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1833422-805602
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1190-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1833422-Animals,
pubmed-meshheading:1833422-Base Sequence,
pubmed-meshheading:1833422-Cells, Cultured,
pubmed-meshheading:1833422-Dinoprostone,
pubmed-meshheading:1833422-Dose-Response Relationship, Drug,
pubmed-meshheading:1833422-Fibroblasts,
pubmed-meshheading:1833422-Humans,
pubmed-meshheading:1833422-Interleukin-1,
pubmed-meshheading:1833422-Liposomes,
pubmed-meshheading:1833422-Mice,
pubmed-meshheading:1833422-Molecular Sequence Data,
pubmed-meshheading:1833422-Oligonucleotides, Antisense,
pubmed-meshheading:1833422-RNA, Messenger,
pubmed-meshheading:1833422-Receptors, Immunologic,
pubmed-meshheading:1833422-Receptors, Interleukin-1
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pubmed:year |
1991
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pubmed:articleTitle |
Oligonucleotides antisense to the interleukin 1 receptor mRNA block the effects of interleukin 1 in cultured murine and human fibroblasts and in mice.
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pubmed:affiliation |
Nova Pharmaceutical Corporation, Baltimore, Maryland 21224.
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pubmed:publicationType |
Journal Article
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