| pubmed-article:1833258 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0023895 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C1442521 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0655410 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:1833258 | lifeskim:mentions | umls-concept:C1447107 | lld:lifeskim |
| pubmed-article:1833258 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1833258 | pubmed:dateCreated | 1991-11-7 | lld:pubmed |
| pubmed-article:1833258 | pubmed:abstractText | For determination of fibroblast-derived tumor cytotoxic factor, F-TCF (human hepatocyte growth factor/hHGF), sensitive two-step sandwich enzyme-linked immunosorbent assay (ELISA) employing monoclonal antibodies was developed. Microplates were coated with monoclonal antibody (P1C8) and bound F-TCF was quantitated with the second monoclonal antibody (P2D6) linked to peroxidase. The standard curve for F-TCF was found to be linear in the range of 0.16 to 10 ng of F-TCF per ml. The assay was specific for F-TCF but not for plasminogen. The assay can be used for determination of F-TCF antigen in both human plasma and serum. The variation of absorbance was little in duplicate samples. Recoveries of exogenous F-TCF added to serum or plasma samples showed theoretical values. F-TCF antigen levels in 21 healthy volunteers was found to be 0.56 +/- 0.43 ng/ml. In contrast, mean F-TCF levels in patients with liver diseases were all higher than those of healthy subjects. This ELISA system has the advantage of using a sensitive and reproducible set of monoclonal antibodies, and is a useful method for monitoring F-TCF levels in patients with liver diseases. | lld:pubmed |
| pubmed-article:1833258 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1833258 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1833258 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1833258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1833258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1833258 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1833258 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1833258 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1833258 | pubmed:issn | 0435-1339 | lld:pubmed |
| pubmed-article:1833258 | pubmed:author | pubmed-author:OkabeKK | lld:pubmed |
| pubmed-article:1833258 | pubmed:author | pubmed-author:ShimaNN | lld:pubmed |
| pubmed-article:1833258 | pubmed:author | pubmed-author:HigashioKK | lld:pubmed |
| pubmed-article:1833258 | pubmed:author | pubmed-author:PICOT | lld:pubmed |
| pubmed-article:1833258 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1833258 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:1833258 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1833258 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1833258 | pubmed:pagination | 477-82 | lld:pubmed |
| pubmed-article:1833258 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:meshHeading | pubmed-meshheading:1833258-... | lld:pubmed |
| pubmed-article:1833258 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1833258 | pubmed:articleTitle | ELISA for F-TCF (human hepatocyte growth factor/hHGF)/fibroblast-derived tumor cytotoxic factor antigen employing monoclonal antibodies and its application to patients with liver diseases. | lld:pubmed |
| pubmed-article:1833258 | pubmed:affiliation | Life Science Research Institute, Snow Brand Milk Products Co., Ltd. Tochigi, Japan. | lld:pubmed |
| pubmed-article:1833258 | pubmed:publicationType | Journal Article | lld:pubmed |
