Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1991-11-7
|
| pubmed:abstractText |
For determination of fibroblast-derived tumor cytotoxic factor, F-TCF (human hepatocyte growth factor/hHGF), sensitive two-step sandwich enzyme-linked immunosorbent assay (ELISA) employing monoclonal antibodies was developed. Microplates were coated with monoclonal antibody (P1C8) and bound F-TCF was quantitated with the second monoclonal antibody (P2D6) linked to peroxidase. The standard curve for F-TCF was found to be linear in the range of 0.16 to 10 ng of F-TCF per ml. The assay was specific for F-TCF but not for plasminogen. The assay can be used for determination of F-TCF antigen in both human plasma and serum. The variation of absorbance was little in duplicate samples. Recoveries of exogenous F-TCF added to serum or plasma samples showed theoretical values. F-TCF antigen levels in 21 healthy volunteers was found to be 0.56 +/- 0.43 ng/ml. In contrast, mean F-TCF levels in patients with liver diseases were all higher than those of healthy subjects. This ELISA system has the advantage of using a sensitive and reproducible set of monoclonal antibodies, and is a useful method for monitoring F-TCF levels in patients with liver diseases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0435-1339
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
477-82
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1833258-Animals,
pubmed-meshheading:1833258-Antibodies, Monoclonal,
pubmed-meshheading:1833258-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1833258-Growth Substances,
pubmed-meshheading:1833258-Hepatocyte Growth Factor,
pubmed-meshheading:1833258-Humans,
pubmed-meshheading:1833258-Liver Diseases,
pubmed-meshheading:1833258-Liver Regeneration,
pubmed-meshheading:1833258-Mice,
pubmed-meshheading:1833258-Mice, Inbred BALB C
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
ELISA for F-TCF (human hepatocyte growth factor/hHGF)/fibroblast-derived tumor cytotoxic factor antigen employing monoclonal antibodies and its application to patients with liver diseases.
|
| pubmed:affiliation |
Life Science Research Institute, Snow Brand Milk Products Co., Ltd. Tochigi, Japan.
|
| pubmed:publicationType |
Journal Article
|