Linked Life Data

1833206

Source:http://linkedlifedata.com/resource/pubmed/id/1833206

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1991-11-13
pubmed:abstractText
We have investigated the role of two signal transduction pathways on the regulation of the gamma and delta T cell antigen receptor (TcR) gene expression, in the acute lymphocytic leukemic cell line DND41. Protein kinase C (PKC) activation, and intracellular free Ca2+ mobilization, initiated by phorbol esters and calcium ionophores, respectively, not only acted independently but, more interestingly, their effects were antagonistic, suggesting a role for these signals during T cell differentiation. The Ca2+ ionophore, ionomycin, increased the levels of intracellular free Ca2+ and induced the expression of the gamma and delta chains of the T cell antigen receptor in a concentration-dependent manner. The phorbol ester 12-myristate 13-acetate down-regulated the basal gamma TcR expression with marginal effect on delta TcR mRNA, but diminished the induction of both gamma and delta TcR, initiated by the Ca2+ ionophore. These antagonistic effects of the two arms of the phospholipase C-mediated signal transduction pathways, i.e. PKC activation and increased intracellular free Ca2+, were specific to the regulation of the gamma and delta TcR, since the same signals exerted a synergistic effect on the mRNA levels of interleukin 2 receptor. These data confirm our hypothesis that the antagonistic regulation on the gamma and delta TcR gene expression by phorbol esters and calcium ionophores occurs in the same cell, and stresses the biological significance of PKC activation and intracellular free calcium mobilization during intrathymic differentiation and selection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2625-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Differential regulation of gamma and delta T cell antigen receptor gene expression by phorbol esters and Ca2+ ionophores in the acute lymphocyte leukemia DND41 cell line.
pubmed:affiliation
Division of Immunology/Rheumatology, Hospital for Sick Children, University of Toronto.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't