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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2008-5-16
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pubmed:abstractText |
JAK2V617F is a common mutation in chronic myeloproliferative diseases (CMPDs). We have developed a system utilizing JAK2V617F-specific guanine quenching probe (QP-system) to detect JAK2V617F. With QP-system, results can be obtained from 100 microl of blood within 90 min. We compared QP-system with direct sequencing using 42 CMPD patients' specimens. JAK2V617F was detected in 25 specimens by QP-system, while direct sequencing failed to detect JAK2V617F in 7 of those 25. The presence of JAK2V617F mutation in these 7 specimens was confirmed by allele-specific PCR. These findings indicate that QP-system is more sensitive and useful than direct sequencing for diagnoses of CMPDs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0145-2126
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pubmed:author |
pubmed-author:AshiharaEishiE,
pubmed-author:HiraiMitsuharuM,
pubmed-author:IshikawaTakayukiT,
pubmed-author:KamitsujiYuriY,
pubmed-author:KawataEriE,
pubmed-author:KimuraShinyaS,
pubmed-author:KobayashiYutakaY,
pubmed-author:KurodaJunyaJ,
pubmed-author:MaekawaTairaT,
pubmed-author:MajimaSatoshiS,
pubmed-author:MurotaniYoshihideY,
pubmed-author:StevensonWilliamW,
pubmed-author:TakeuchiMikiM,
pubmed-author:TakiTomohikoT,
pubmed-author:TanakaRurikoR,
pubmed-author:TaniwakiMasafumiM,
pubmed-author:YokotaAsumiA
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pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1462-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18328559-Adult,
pubmed-meshheading:18328559-Aged,
pubmed-meshheading:18328559-Aged, 80 and over,
pubmed-meshheading:18328559-Automation,
pubmed-meshheading:18328559-Chronic Disease,
pubmed-meshheading:18328559-DNA Mutational Analysis,
pubmed-meshheading:18328559-DNA Primers,
pubmed-meshheading:18328559-Female,
pubmed-meshheading:18328559-Humans,
pubmed-meshheading:18328559-Janus Kinase 2,
pubmed-meshheading:18328559-Male,
pubmed-meshheading:18328559-Middle Aged,
pubmed-meshheading:18328559-Myeloproliferative Disorders,
pubmed-meshheading:18328559-Point Mutation,
pubmed-meshheading:18328559-Polymerase Chain Reaction
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pubmed:year |
2008
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pubmed:articleTitle |
Fully automated and super-rapid system for the detection of JAK2V617F mutation.
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pubmed:affiliation |
Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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