Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-10-24
pubmed:abstractText
A cDNA encoding the nonmuscle-specific (type B) subunit of phosphoglycerate mutase (PGAM-B) was isolated and characterized. A cDNA probe, synthesized by the polymerase chain reaction (PCR) from rat liver cell mRNA using mixed primers specific to the amino acid sequence of human PGAM-B, was used to screen a rat liver cell cDNA library. The identity of the cDNA was confirmed by amino acid sequence data for 24 peptides obtained by digesting the purified protein with three different endopeptidases. The coding region encoded a polypeptide composed of 253 amino acid (plus the initiator Met). RNA blot analysis showed a single mRNA species of 1.7 kilobases in rat liver cell. The deduced amino acid sequence of rat PGAM-B was identical to that of human PGAM-B except for only one substitution at position 251 near the carboxyl terminus (valine for the rat and alanine for the human).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
558-61
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1832843-Amino Acid Sequence, pubmed-meshheading:1832843-Animals, pubmed-meshheading:1832843-Base Sequence, pubmed-meshheading:1832843-Bisphosphoglycerate Mutase, pubmed-meshheading:1832843-Cell Line, pubmed-meshheading:1832843-Cloning, Molecular, pubmed-meshheading:1832843-DNA, pubmed-meshheading:1832843-Gene Library, pubmed-meshheading:1832843-Humans, pubmed-meshheading:1832843-Isoenzymes, pubmed-meshheading:1832843-Liver, pubmed-meshheading:1832843-Macromolecular Substances, pubmed-meshheading:1832843-Molecular Sequence Data, pubmed-meshheading:1832843-Oligonucleotide Probes, pubmed-meshheading:1832843-Polymerase Chain Reaction, pubmed-meshheading:1832843-Rats, pubmed-meshheading:1832843-Rats, Inbred BUF, pubmed-meshheading:1832843-Restriction Mapping, pubmed-meshheading:1832843-Sequence Homology, Nucleic Acid
pubmed:year
1991
pubmed:articleTitle
cDNA encoding type B subunit of rat phosphoglycerate mutase: its isolation and nucleotide sequence.
pubmed:affiliation
Nagahama Institute for Biochemical Science, Oriental Yeast Company, Ltd., Shiga, Japan.
pubmed:publicationType
Journal Article, Comparative Study