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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2008-3-7
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pubmed:abstractText |
Ferredoxin-NAD(P)(+) reductase (FNR) is a key enzyme that catalyzes the photoreduction of NAD(P)(+) to generate NAD(P)H during the final step of the photosynthetic electron-transport chain. FNR from the green sulfur bacterium Chlorobium tepidum is a homodimeric enzyme with a molecular weight of 90 kDa; it shares a high level of amino-acid sequence identity to thioredoxin reductase rather than to conventional plant-type FNRs. In order to understand the structural basis of the ferredoxin-dependency of this unique photosynthetic FNR, C. tepidum FNR has been heterologously expressed, purified and crystallized in two forms. Form I crystals belong to space group C222(1) and contain one dimer in the asymmetric unit, while form II crystals belong to space group P4(1)22 or P4(3)22. Diffraction data were collected from a form I crystal to 2.4 A resolution on the synchrotron-radiation beamline NW12 at the Photon Factory.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-10369776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-10467097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-10595539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-11175898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-11243797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-11300769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-12009411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-12093901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-15252706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-16217027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-16301794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-1986412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-8034707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-8108411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-8114095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-8533008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-9149148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18323604-9865948
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
186-9
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pubmed:dateRevised |
2010-9-21
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum.
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pubmed:affiliation |
Department of Life Sciences, University of Tokyo, Komaba, Tokyo 153-8902, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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