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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-10-11
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pubmed:databankReference |
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pubmed:abstractText |
SWI4 of budding yeast codes for a component of a transcription factor (cell cycle box factor, or CCBF) necessary for G1-specific expression of HO. We show that SWI4 is essential for haploid cell viability at high temperature and in a/alpha cells at all temperatures: SWI4-deficient cells arrest as large unbudded cells. Eight high copy number plasmids were identified that allow swi4- strains to grow under nonpermissive conditions. Two carry G1 cyclin genes, CLN1 and CLN2; another carries HCS26, coding for a putative cyclin, a/alpha swi4- mutants exhibit 3- to 20-fold reductions in the levels of CLN1, CLN2, and HCS26 transcripts. The requirement of SWI4 for transcription appears to be direct: each gene contains sites similar to the CCBF-binding site; CCBF binds to the upstream region of HCS26. We propose that SWI4 participates in a positive feedback loop by which CLN1, CLN2, and possibly HCS26 promote their own transcription in G1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1015-26
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1832336-Amino Acid Sequence,
pubmed-meshheading:1832336-Base Sequence,
pubmed-meshheading:1832336-Cell Cycle,
pubmed-meshheading:1832336-Cloning, Molecular,
pubmed-meshheading:1832336-Cyclins,
pubmed-meshheading:1832336-DNA-Binding Proteins,
pubmed-meshheading:1832336-Fungal Proteins,
pubmed-meshheading:1832336-Gene Expression Regulation, Fungal,
pubmed-meshheading:1832336-Genes, Lethal,
pubmed-meshheading:1832336-Genes, Suppressor,
pubmed-meshheading:1832336-Macromolecular Substances,
pubmed-meshheading:1832336-Molecular Sequence Data,
pubmed-meshheading:1832336-Protein Binding,
pubmed-meshheading:1832336-RNA, Fungal,
pubmed-meshheading:1832336-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1832336-Saccharomyces cerevisiae,
pubmed-meshheading:1832336-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:1832336-Transcription, Genetic,
pubmed-meshheading:1832336-Transcription Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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