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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1991-10-4
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pubmed:abstractText |
We have studied interactions between bacterially produced E1A linked to Sepharose and the various DNA-binding proteins present in HeLa cell nuclear extracts (NE). DNA-binding activities and cross-reactive polypeptides recognizing the cAMP-responsive element (CRE) and the activator protein 1 (AP1) sites were bound to the E1A column, whereas nuclear factor 1 (NF1) and the activator protein 2 (AP2) DNA-binding activities were not retained by E1A. The binding activities that were retained belonged to the CRE and JUN protein family, as judged by Western blot analysis. Authentic CRE-BP1, c-Jun and c-Fos proteins produced by in-vitro translation also bound to the E1A column. However, efficient binding of in-vitro-translated CRE-BP1 and c-Fos proteins to E1A required preincubation with NE. We show here that immobilized E1A sequesters several cellular upstream transcription activators, and suggest a role for members of the AP1 family of transcription factors in E1A-mediated gene regulation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1417-22
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1832215-Adenovirus Early Proteins,
pubmed-meshheading:1832215-Blotting, Western,
pubmed-meshheading:1832215-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:1832215-DNA-Binding Proteins,
pubmed-meshheading:1832215-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:1832215-HeLa Cells,
pubmed-meshheading:1832215-Humans,
pubmed-meshheading:1832215-Oncogene Proteins, Viral,
pubmed-meshheading:1832215-Protein Biosynthesis,
pubmed-meshheading:1832215-Proto-Oncogene Proteins,
pubmed-meshheading:1832215-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:1832215-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:1832215-Transcription Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Interactions between adenovirus E1A and members of the AP-1 family of cellular transcription factors.
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pubmed:affiliation |
Wistar Institute, Philadelphia, Pennsylvania 19104.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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