|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0086418,
umls-concept:C0162638,
umls-concept:C0205314,
umls-concept:C0334227,
umls-concept:C0679622,
umls-concept:C0684249,
umls-concept:C1155873,
umls-concept:C1156236,
umls-concept:C1412251,
umls-concept:C1611034,
umls-concept:C2917322
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2008-5-6
|
|
pubmed:abstractText |
Human A3 adenosine receptor (A3AR) agonists showed the anti-tumor activity in various in vitro and in vivo studies. The present study investigates the anti-proliferative effect of a novel adenosine analog 2-chloro-N6-(3-iodobenzyl)-4'-thioadenosine-5'-N-methyluronamide (thio-Cl-IB-MECA) in A549 human lung cancer cells. Thio-Cl-IB-MECA induced arrest of cell cycle progression in G0/G1 phase at lower concentrations (up to 20 microM) and apoptotic cell death at a higher concentration (80 microM), which were manifested by down-regulation of cyclin D1, c-myc, and CDK4, activation of caspase-3 and -9, and cleavage of poly(ADP-ribose) polymerase (PARP). The activation of Akt-mediated signaling was also inhibited by treatment with thio-Cl-IB-MECA. These data might suggest the potential therapeutic value of an adenosine analog in the treatment of human lung cancer.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0304-3835
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
18
|
|
pubmed:volume |
264
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
309-15
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18321638-Adenosine,
pubmed-meshheading:18321638-Adenosine A3 Receptor Agonists,
pubmed-meshheading:18321638-Antineoplastic Agents,
pubmed-meshheading:18321638-Apoptosis,
pubmed-meshheading:18321638-Blotting, Western,
pubmed-meshheading:18321638-Caspases,
pubmed-meshheading:18321638-Cell Cycle,
pubmed-meshheading:18321638-Cell Line, Tumor,
pubmed-meshheading:18321638-Cell Proliferation,
pubmed-meshheading:18321638-Enzyme Activation,
pubmed-meshheading:18321638-Gene Expression,
pubmed-meshheading:18321638-Humans,
pubmed-meshheading:18321638-Lung Neoplasms,
pubmed-meshheading:18321638-Proto-Oncogene Proteins c-akt
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Inhibition of cell proliferation through cell cycle arrest and apoptosis by thio-Cl-IB-MECA, a novel A3 adenosine receptor agonist, in human lung cancer cells.
|
|
pubmed:affiliation |
College of Pharmacy, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-ku, Seoul 120-750, Republic of Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
