| pubmed-article:18317988 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C0012265 | lld:lifeskim |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C0809874 | lld:lifeskim |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:18317988 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:18317988 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:18317988 | pubmed:dateCreated | 2008-6-30 | lld:pubmed |
| pubmed-article:18317988 | pubmed:abstractText | Digoxin, a commonly prescribed cardiac glycoside with a narrow therapeutic window, is routinely used in pharmacokinetic studies to assess the in vivo activity of the drug efflux pump P-glycoprotein. To minimize adverse events, a sub-therapeutic dose of digoxin is usually administered, producing low plasma concentrations requiring a sensitive detection technique. Commonly available immunoassay techniques do not provide the required sensitivity to measure these low plasma concentrations and are potentially non-specific in certain subject populations. Previously published mass spectrometric techniques require either large plasma volumes or a tandem mass spectrometer. To overcome these challenges we have developed a sensitive and specific LC-MS method for the quantification of digoxin in small volumes of human plasma and urine. Plasma (1 mL) was extracted with methyl t-butyl ether under basic conditions followed by LC-MS detection of the sodium adducts of digoxin (803.4 m/z) and digitoxin (787.4 m/z, internal standard). Linearity and accuracy were demonstrated across a wide range of digoxin plasma concentration (0.05-1.5 ng/mL). This specific, sensitive, validated digoxin LC-MS assay can be used to quantify sub-therapeutic digoxin plasma concentrations in men and women (pregnant and non-pregnant). | lld:pubmed |
| pubmed-article:18317988 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18317988 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18317988 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18317988 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18317988 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:18317988 | pubmed:issn | 0269-3879 | lld:pubmed |
| pubmed-article:18317988 | pubmed:author | pubmed-author:UnadkatJashva... | lld:pubmed |
| pubmed-article:18317988 | pubmed:author | pubmed-author:KirbyBrian... | lld:pubmed |
| pubmed-article:18317988 | pubmed:author | pubmed-author:HebertMaryM | lld:pubmed |
| pubmed-article:18317988 | pubmed:author | pubmed-author:KalhornTomT | lld:pubmed |
| pubmed-article:18317988 | pubmed:author | pubmed-author:EasterlingTom... | lld:pubmed |
| pubmed-article:18317988 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18317988 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:18317988 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18317988 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18317988 | pubmed:pagination | 712-8 | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:meshHeading | pubmed-meshheading:18317988... | lld:pubmed |
| pubmed-article:18317988 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18317988 | pubmed:articleTitle | Sensitive and specific LC-MS assay for quantification of digoxin in human plasma and urine. | lld:pubmed |
| pubmed-article:18317988 | pubmed:affiliation | Department of Pharmaceutics, University of Washington, Seattle, WA 98195, USA. | lld:pubmed |
| pubmed-article:18317988 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18317988 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| pubmed-article:18317988 | pubmed:publicationType | Validation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18317988 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18317988 | lld:pubmed |
