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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-10-3
|
| pubmed:databankReference | |
| pubmed:abstractText |
The Schizosaccharomyces pombe nuclear gene, atp2, encoding the beta subunit of the mitochondrial ATP synthase, was sequenced and found to contain a 1575-bp open reading frame. Two adjacent transcription-initiation sites were found at positions 34 and 44 nucleotides upstream of the translation-initiation codon. The deduced polypeptide sequence was composed of 525 amino acid residues (molecular mass = 56875 Da). The mature polypeptide starts at residue 45 (molecular mass = 51,685 Da), indicating the presence of a presequence of 44 residues, presumably involved in mitochondrial targeting. The atp2 mutant B59-1 [Boutry, M. & Goffeau, A. (1982) Eur. J. Biochem. 125, 471-477] and its related revertant allele R4-3 [Jault, J. M., Di Pietro, A., Falson, P., Gautheron, D. C., Boutry, M. & Goffeau, A. (1989) Biochem. Biophys. Res. Commun. 158, 392-399] were also cloned and sequenced. A single nonsense mutation, CAG (Gln170)----TAG (stop) in mutant B59-1, became a missense mutation, TAG (stop)----TAC (Tyr) in revertant R4-3. Gln170 is located between the first and second elements belonging to the nucleotide-binding site. Its substitution by a tyrosine residue increases the enzyme affinity towards ADP, the amount of endogenous nucleotides and the apparent negative cooperativity for ATPase activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
200
|
| pubmed:geneSymbol |
atp2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
61-7
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:1831760-Alleles,
pubmed-meshheading:1831760-Amino Acid Sequence,
pubmed-meshheading:1831760-Base Sequence,
pubmed-meshheading:1831760-DNA-Binding Proteins,
pubmed-meshheading:1831760-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1831760-Genes, Fungal,
pubmed-meshheading:1831760-Mitochondria,
pubmed-meshheading:1831760-Molecular Sequence Data,
pubmed-meshheading:1831760-Mutation,
pubmed-meshheading:1831760-Open Reading Frames,
pubmed-meshheading:1831760-Polymerase Chain Reaction,
pubmed-meshheading:1831760-Proton-Translocating ATPases,
pubmed-meshheading:1831760-Schizosaccharomyces,
pubmed-meshheading:1831760-Transcription Factors
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Beta subunit of mitochondrial F1-ATPase from the fission yeast. Deduced sequence of the wild type protein and identification of a mutation that increases nucleotide binding.
|
| pubmed:affiliation |
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-La-Neuve, Belgium.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|