Linked Life Data

18314909

Source:http://linkedlifedata.com/resource/pubmed/id/18314909

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-4-8
pubmed:abstractText
Amplification at the 11q13 locus is commonly observed in breast, ovarian, head and neck, oral, and esophageal cancer. Studies of this region led to the identification of multiple amplicons containing several potential oncogenes including EMSY, PAK1, RSF1, and GAB2. Here, we investigate the amplification of the above four genes and their prognostic significance in histologically and clinically defined subsets of ovarian cancer. Amplification of all four genes was assessed by fluorescent in situ hybridization in tissue microarrays containing 538 clinically annotated ovarian carcinomas with 12 years of follow-up data. Overall, for the entire cohort, EMSY was amplified in 44 (16%) of 269 cases, PAK1 was amplified in 38 (15%) of 255 cases, RSF1 was amplified in 37 (12%) of 310 cases, and GAB2 was amplified in 41 (16%) of 255 cases. Amplification of EMSY, PAK1, RSF1, and GAB2 were all highly correlated with each other and with a serous histology. Univariate survival analysis showed that tumors with EMSY and RSF1 amplification were associated with a significantly worse outcome. A molecular inversion probe array was then used to study the 11q13 amplicon in 33 high grade serous carcinomas. The core of the amplicon mapped to a 6-Mb region encompassing EMSY, PAK1, RSF1, and GAB2. However, a second more telomeric amplicon was also observed for which no candidate genes have been identified. In summary, amplification of these four putative oncogenes from 11q13 in early ovarian cancer is associated with a serous histology and in the case of EMSY and RSF1 a poor outcome. These findings support the hypothesis that the11q13 amplicon in ovarian cancer is likely driven by a cassette of genes rather than by a single oncogene. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1098-2264
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
481-9
pubmed:dateRevised
2011-10-13
pubmed:meshHeading
pubmed-meshheading:18314909-Adaptor Proteins, Signal Transducing, pubmed-meshheading:18314909-Carcinoma, pubmed-meshheading:18314909-Chromosomes, Human, Pair 11, pubmed-meshheading:18314909-Cystadenocarcinoma, Serous, pubmed-meshheading:18314909-Female, pubmed-meshheading:18314909-Gene Amplification, pubmed-meshheading:18314909-Humans, pubmed-meshheading:18314909-In Situ Hybridization, Fluorescence, pubmed-meshheading:18314909-Multigene Family, pubmed-meshheading:18314909-Neoplasm Proteins, pubmed-meshheading:18314909-Nuclear Proteins, pubmed-meshheading:18314909-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:18314909-Oncogenes, pubmed-meshheading:18314909-Ovarian Neoplasms, pubmed-meshheading:18314909-Prognosis, pubmed-meshheading:18314909-Repressor Proteins, pubmed-meshheading:18314909-Trans-Activators, pubmed-meshheading:18314909-p21-Activated Kinases
pubmed:year
2008
pubmed:articleTitle
Amplification of 11q13 in ovarian carcinoma.
pubmed:affiliation
Department of Pathology, Vancouver Coastal Health Research Institute, Genetic Pathology Evaluation Centre, British Columbia Cancer Agency, University of British Columbia, Vancouver, BC, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't