Linked Life Data

18313777

Source:http://linkedlifedata.com/resource/pubmed/id/18313777

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-3-21
pubmed:abstractText
We developed and tested a method to produce DNA standards and controls for quantitative PCR by designing and performing partial hybridization of long oligonucleotides before double stranded DNA fragments were synthesized and subsequently amplified by conventional PCR. This approach does not require any natural DNA template. Applications include the production of standards, which cannot be easily produced from DNA extracted from bacteria or plants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0167-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-7
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A novel and rapid method for synthesizing positive controls and standards for quantitative PCR.
pubmed:affiliation
Environmental Microbial Genomics Group, Microsystems and Microbiology, Laboratoire Ampère, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue 69134 Ecully cedex, France.
pubmed:publicationType
Journal Article, Evaluation Studies