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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1991-9-18
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pubmed:abstractText |
Transforming growth factor beta 1 (TGF-beta 1) is known to inhibit the growth of immature hematopoietic progenitor cells, whereas more mature, lineage-restricted progenitors are not inhibited. In contrast, in the presence of saturating concentrations of granulocyte/macrophage-colony-stimulating factor (GM-CSF), TGF-beta promoted a 3- to 5-fold increase in the number and size (greater than 0.5 mm) of bone marrow colonies in a dose-dependent manner with an ED50 of 10-20 pM; TGF-beta 1 alone had no effect. Morphological examination showed an increase in granulocyte colonies. In suspension cultures, TGF-beta 1 and GM-CSF stimulated an increase in total viable cells with markedly enhanced neutrophilic differentiation and a concomitant decrease in the number of monocytes/macrophages by day 6 in culture. Limiting dilution analysis demonstrated a 2- to 5-fold increase in the frequency of progenitor cells that responded to GM-CSF plus TGF-beta 1 vs. GM-CSF alone. Bone marrow progenitors obtained from mice 3 days after treatment with 5-fluorouracil responded to a combination of GM-CSF and TGF-beta 1, whereas either factor alone had no effect. A single-cell assay identified a progenitor cell that directly responded to TGF-beta and GM-CSF. TGF-beta increased the number of GM-CSF receptors on bone marrow cells. Thus, TGF-beta 1 can act as a bifunctional mediator of hematopoietic cell growth, and TGF-beta 1 and GM-CSF act together to stimulate granulopoiesis as measured by large granulocyte colony formation; the progenitor cell is tentatively designated granulocyte burst-forming unit.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-1707695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-1967539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2189013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2460153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2549855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2745976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2828856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2889143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2898810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-2981896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3064207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3258618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3261777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3472612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3488321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3528157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-3871521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-4048193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-4822532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-5021304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-6690617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1831268-6815268
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7190-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1831268-Animals,
pubmed-meshheading:1831268-Bone Marrow,
pubmed-meshheading:1831268-Bone Marrow Cells,
pubmed-meshheading:1831268-Cell Division,
pubmed-meshheading:1831268-Cell Survival,
pubmed-meshheading:1831268-Cells, Cultured,
pubmed-meshheading:1831268-Colony-Stimulating Factors,
pubmed-meshheading:1831268-Drug Synergism,
pubmed-meshheading:1831268-Fluorouracil,
pubmed-meshheading:1831268-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1831268-Granulocytes,
pubmed-meshheading:1831268-Hematopoiesis,
pubmed-meshheading:1831268-Hematopoietic Stem Cells,
pubmed-meshheading:1831268-Interleukins,
pubmed-meshheading:1831268-Kinetics,
pubmed-meshheading:1831268-Macrophages,
pubmed-meshheading:1831268-Mice,
pubmed-meshheading:1831268-Receptors, Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1831268-Recombinant Proteins,
pubmed-meshheading:1831268-Transforming Growth Factor beta
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pubmed:year |
1991
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pubmed:articleTitle |
Stimulation of granulopoiesis by transforming growth factor beta: synergy with granulocyte/macrophage-colony-stimulating factor.
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pubmed:affiliation |
Biological Carcinogenesis and Development Program, Program Resources, Inc., Frederick, MD.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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