Linked Life Data

18310485

Source:http://linkedlifedata.com/resource/pubmed/id/18310485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-5-21
pubmed:abstractText
The aim of this note is to describe a reliable, fast, and cost-effective real-time PCR method for routine genotyping of mutations responsible for most coat color variation in horses. The melanocortin-1 receptor, Agouti-signaling peptide, and membrane-associated transporter protein alleles were simultaneously determined using 2 PCR protocols. The assay described here is an alternative method for routine genotyping of a defined number of polymorphisms. Allelic variants are detected in real time and no post-PCR manipulations are required, therefore limiting costs and possible carryover contamination. Data can be copied to a Microsoft Excel spreadsheet for semiautomatic determination of the genotype using a macro freely available at http://www.igijon.com/personales/fgoyache/software_i.htm (last accessed February 26, 2007). The performance of the method is demonstrated on 156 Spanish Purebred horses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1525-3163
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1291-5
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Technical note: a novel method for routine genotyping of horse coat color gene polymorphisms.
pubmed:affiliation
Servicio Regional de Investigación y Desarrollo Agroalimentario del Principado de Asturias-Somió, C/Camino de los Claveles 604, E-33203 Gijón (Asturias), Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't