Linked Life Data

18305374

Source:http://linkedlifedata.com/resource/pubmed/id/18305374

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-2-28
pubmed:abstractText
We reported the human flavin-containing monooxygenase 3 (FMO3) haplotypes (Pharmacogenet. Genomics: 17, 827, 2007). The objective was to gain the insight into transcriptional regulation in a Japanese population. The wild-type FMO3 reporter plasmids carrying 5'-flanking sequence from the transcriptional initiation site of the FMO3 haplotype 1 (prepared from three individuals) showed higher luciferase activities in HepG2 cells than those from the FMO3 haplotypes 2 and 3, with the wild-type coding region. Several deletion mutants of the FMO3 haplotype 1 (extending from -5,167 to -1,764, numbered relative to the A of the ATG translational initiation codon) revealed that the region of -2,064 to -1,804 contained an important cis-acting element(s) for activation of the FMO3 gene expression. Putative hepatocyte nuclear factor-4 (HNF-4) binding site and CCAAT box, but not Yin Yang 1 element, could be responsible cis-acting elements of the FMO3 gene, by site-directed mutagenesis analysis. The unknown suppressive cis-element(s) at the 5'-upstream region from -2,064 might show genetic polymorphism, because the FMO3 haplotypes 2 and 3 had three and ten mutations, respectively. These results suggest that the putative HNF-4 binding site and CCAAT box could be responsible cis-acting elements of the FMO3 gene in Japanese.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1880-0920
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
54-8
pubmed:meshHeading
pubmed-meshheading:18305374-5' Untranslated Regions, pubmed-meshheading:18305374-Amino Acid Motifs, pubmed-meshheading:18305374-Asian Continental Ancestry Group, pubmed-meshheading:18305374-Base Sequence, pubmed-meshheading:18305374-Binding Sites, pubmed-meshheading:18305374-Cell Line, Tumor, pubmed-meshheading:18305374-Gene Expression Regulation, Enzymologic, pubmed-meshheading:18305374-Haplotypes, pubmed-meshheading:18305374-Hepatocyte Nuclear Factor 4, pubmed-meshheading:18305374-Humans, pubmed-meshheading:18305374-Molecular Sequence Data, pubmed-meshheading:18305374-Mutagenesis, Site-Directed, pubmed-meshheading:18305374-Oxygenases, pubmed-meshheading:18305374-Polymorphism, Genetic, pubmed-meshheading:18305374-Response Elements, pubmed-meshheading:18305374-Suppression, Genetic, pubmed-meshheading:18305374-Transcription, Genetic
pubmed:year
2008
pubmed:articleTitle
Complex mechanism underlying transcriptional control of the haplotyped flavin-containing monooxygenase 3 (FMO3) gene in Japanese: different regulation between mutations in 5'-upstream distal region and common element in proximal region.
pubmed:affiliation
Laboratory of Drug Metabolism and Pharmacokinetics, Showa Pharmaceutical University, Machida, Tokyo, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't