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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-28
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pubmed:abstractText |
To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1365-3156
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pubmed:author |
pubmed-author:AblordeyAnthonyA,
pubmed-author:AffolabiDissouD,
pubmed-author:AguiarJuliaJ,
pubmed-author:AnagonouSéverinS,
pubmed-author:BankoléHonoréH,
pubmed-author:DossouAngeA,
pubmed-author:HounnougaJulieJ,
pubmed-author:JohnsonRoch ChristianRC,
pubmed-author:KoutchakpoPierreP,
pubmed-author:PortaelsFrançoiseF,
pubmed-author:SopohGhislainG
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pubmed:issnType |
Electronic
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-90
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pubmed:meshHeading |
pubmed-meshheading:18304264-Bacteriological Techniques,
pubmed-meshheading:18304264-Benzophenoneidum,
pubmed-meshheading:18304264-Buruli Ulcer,
pubmed-meshheading:18304264-Coloring Agents,
pubmed-meshheading:18304264-DNA Transposable Elements,
pubmed-meshheading:18304264-Humans,
pubmed-meshheading:18304264-Microscopy,
pubmed-meshheading:18304264-Mycobacterium ulcerans,
pubmed-meshheading:18304264-Polymerase Chain Reaction,
pubmed-meshheading:18304264-Rosaniline Dyes,
pubmed-meshheading:18304264-Sensitivity and Specificity,
pubmed-meshheading:18304264-Specimen Handling,
pubmed-meshheading:18304264-Staining and Labeling,
pubmed-meshheading:18304264-Subcutaneous Tissue
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pubmed:year |
2008
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pubmed:articleTitle |
Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis.
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pubmed:affiliation |
Laboratoire de Référence des Mycobactéries, Cotonou, Bénin, and Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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