Source:http://linkedlifedata.com/resource/pubmed/id/18303029
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
18
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| pubmed:dateCreated |
2008-4-28
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| pubmed:abstractText |
To understand the tumor-suppressing mechanism of the SWI/SNF chromatin remodeling complex, we investigated its molecular relationship with p53. Using the pREP4-luc episomal reporter, we first demonstrated that p53 utilizes the chromatin remodeling activity of the SWI/SNF complex to initiate transcription from the chromatin-structured promoter. Among the components of the SWI/SNF complex, we identified BAF60a as a mediator of the interaction with p53 by the yeast two-hybrid assay. p53 directly interacted only with BAF60a, but not with other components of the SWI/SNF complex, such as BRG1, SRG3, SNF5, or BAF57. We found out that multiple residues at the amino acid 108-150 region of BAF60a were involved in the interaction with the tetramerization domain of p53. The N-terminal fragment of BAF60a containing the p53-interacting region as well as small interfering RNA for baf60a inhibited the SWI/SNF complex-mediated transcriptional activity of p53. The uncoupling of p53 with the SWI/SNF complex resulted in the repression of both p53-dependent apoptosis and cell cycle arrest by the regulation of target genes. These results suggest that the SWI/SNF chromatin remodeling complex is involved in the suppression of tumors by the interaction with p53.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Mutant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SWI-SNF-B chromatin-remodeling...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
2
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| pubmed:volume |
283
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
11924-34
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| pubmed:dateRevised |
2011-11-7
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| pubmed:meshHeading |
pubmed-meshheading:18303029-Amino Acid Sequence,
pubmed-meshheading:18303029-Animals,
pubmed-meshheading:18303029-Apoptosis,
pubmed-meshheading:18303029-Cell Cycle,
pubmed-meshheading:18303029-Cell Line,
pubmed-meshheading:18303029-Chromatin Assembly and Disassembly,
pubmed-meshheading:18303029-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:18303029-Down-Regulation,
pubmed-meshheading:18303029-Doxorubicin,
pubmed-meshheading:18303029-Humans,
pubmed-meshheading:18303029-Mice,
pubmed-meshheading:18303029-Mutant Proteins,
pubmed-meshheading:18303029-Protein Binding,
pubmed-meshheading:18303029-Protein Interaction Mapping,
pubmed-meshheading:18303029-Protein Structure, Tertiary,
pubmed-meshheading:18303029-Transcription, Genetic,
pubmed-meshheading:18303029-Transcription Factors,
pubmed-meshheading:18303029-Transduction, Genetic,
pubmed-meshheading:18303029-Tumor Suppressor Protein p53
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| pubmed:year |
2008
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| pubmed:articleTitle |
BAF60a interacts with p53 to recruit the SWI/SNF complex.
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| pubmed:affiliation |
Department of Biological Sciences, Institute of Molecular Biology and Genetics, Research Center for Functional Cellulomics, Seoul National University, Seoul 151-742, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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