Linked Life Data

18301881

Source:http://linkedlifedata.com/resource/pubmed/id/18301881

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-7-29
pubmed:abstractText
An ordinary atomic force microscopy (AFM) was functionalized and applied to electrochemically draw micropatterns of biomolecules. To fabricate an electrochemical AFM probe having an electrode at the tip, a metal-coated AFM probe was first insulated with Parylene C, and then the apex of the tip was ground mechanically to expose the electrode. The effective electrode diameter was estimated to be ca. 500 nm. The electrode probe was positioned close to a heparin-coated antibiofouling substrate and used to locally generate hypobromous acid from a dilute Br(-) solution to render the substrate surface protein-adhesive. In situ topographical imaging after the electrochemical treatment suggested the heparin layer became detached to allow the adsorption of proteins, in this case fibronectin. The diameter of the drawn fibronectin pattern was 2 microm, which is one order of magnitude smaller than we achieved previously using a microdisk electrode (tip diameter 10 microm).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1618-2650
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
391
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2711-6
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Integration of an electrochemical-based biolithography technique into an AFM system.
pubmed:affiliation
Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai, 980-8579, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't