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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-2-26
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pubmed:abstractText |
T-bet, a Th1-specific transcription factor, can promote the production of IFN-gamma. IFN-gamma is the principal Th1 effector cytokine and it has a crucial role in Th1 differentiation, which can drive the differentiation of naïve CD4+T cells into T-helper 1 (Th1) cells. In our study, a human T-bet gene was fused with a gene fragment encoding HIV-1 protein transduction domain in a bacterial expression vector to produce a Tat/T-bet fusion protein. The expressed and purified Tat/T-bet proteins were transduced efficiently into THP-1 cells in a time- and dose-dependent manner; when Tat/T-bet pretreated THP-1 cells were co-cultured with CD4+T cells, the IFN-gamma level increased higher to about 7 pg/ml, 10-folds as compared with the normal level when tested at 48 hours. The results demonstrated that the Tat/T-bet fusion protein can be efficiently transduced into antigen-presenting cells (APCs) like THP-1 cells and then regulated Th1/Th2 balance, which may act as a potential tool for gene therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1532-4311
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pubmed:author |
pubmed-author:DingQingQ,
pubmed-author:LobMM,
pubmed-author:MaBingB,
pubmed-author:MaoChaomingC,
pubmed-author:QiuGufengG,
pubmed-author:ShaoQixiangQ,
pubmed-author:TongJiaJ,
pubmed-author:WangShengjunS,
pubmed-author:WangSuoyingS,
pubmed-author:XuHuaxiH,
pubmed-author:XuXiaopengX,
pubmed-author:YangMinM
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pubmed:issnType |
Electronic
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-111
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18300036-Antigen-Presenting Cells,
pubmed-meshheading:18300036-Blotting, Western,
pubmed-meshheading:18300036-Cell Differentiation,
pubmed-meshheading:18300036-Cell Line,
pubmed-meshheading:18300036-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18300036-Flow Cytometry,
pubmed-meshheading:18300036-Gene Therapy,
pubmed-meshheading:18300036-Genes, tat,
pubmed-meshheading:18300036-Genetic Vectors,
pubmed-meshheading:18300036-HIV,
pubmed-meshheading:18300036-Humans,
pubmed-meshheading:18300036-Interferon-gamma,
pubmed-meshheading:18300036-Lymphocyte Activation,
pubmed-meshheading:18300036-Recombinant Fusion Proteins,
pubmed-meshheading:18300036-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18300036-T-Box Domain Proteins,
pubmed-meshheading:18300036-Th1 Cells,
pubmed-meshheading:18300036-Th2 Cells,
pubmed-meshheading:18300036-Transduction, Genetic
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pubmed:year |
2008
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pubmed:articleTitle |
Tat-mediated intracellular delivery of T-bet protein into THP-1 cells can induce Th1-type response.
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pubmed:affiliation |
Department of Immunology, School of Medical Technology, Jiangsu University, Zhenjiang, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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