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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2008-4-18
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pubmed:abstractText |
The objectives of the present work were to assess whether epithelial cells from the different segments of epididymis express TR alpha 1-beta 1 isoforms, to depict its subcellular immunolocalization and to evaluate changes in their expression in rats experimentally submitted to a hypothyroid state by injection of 131I. In euthyroid and hypothyroid groups, TR protein was expressed in epididymal epithelial cells, mainly in the cytoplasmic compartment while only a few one showed a staining in the nucleus as well. A similar TR immunostaining pattern was detected in the different segments of the epididymis. In hypothyroid rats, the number of TR-immunoreactive epithelial cells as well as the intensity of the cytoplasmic staining significantly increased in all sections analyzed. In consonance to the immunocytochemical analysis, the expression of TR alpha 1-beta 1 isoforms, assessed by Western blot revealed significantly higher levels of TR in cytosol compared to the nuclear fractions. Furthermore, TR expression of both alpha 1 and beta 1 isoforms and their mRNA levels were increased by the hypothyroid state. The immuno-electron-microscopy showed specific reaction for TR in principal cells associated with eucromatin, cytosolic matrix and mitochondria. The differences in expression levels assessed in control and thyroidectomized rats ascertain a specific function of TH on this organ.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0948-6143
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
631-42
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pubmed:meshHeading |
pubmed-meshheading:18299881-Animals,
pubmed-meshheading:18299881-Blotting, Western,
pubmed-meshheading:18299881-Cell Nucleus,
pubmed-meshheading:18299881-Cytoplasm,
pubmed-meshheading:18299881-Epididymis,
pubmed-meshheading:18299881-Epithelial Cells,
pubmed-meshheading:18299881-Gene Expression,
pubmed-meshheading:18299881-Hypothyroidism,
pubmed-meshheading:18299881-Immunohistochemistry,
pubmed-meshheading:18299881-Male,
pubmed-meshheading:18299881-Microscopy, Immunoelectron,
pubmed-meshheading:18299881-RNA, Messenger,
pubmed-meshheading:18299881-Rats,
pubmed-meshheading:18299881-Rats, Wistar,
pubmed-meshheading:18299881-Receptors, Thyroid Hormone,
pubmed-meshheading:18299881-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18299881-Thyroid Gland,
pubmed-meshheading:18299881-Thyroid Hormone Receptors alpha,
pubmed-meshheading:18299881-Thyroid Hormone Receptors beta,
pubmed-meshheading:18299881-Thyroxine,
pubmed-meshheading:18299881-Triiodothyronine
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pubmed:year |
2008
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pubmed:articleTitle |
Thyroid hormone receptor alpha 1-beta 1 expression in epididymal epithelium from euthyroid and hypothyroid rats.
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pubmed:affiliation |
Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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