Linked Life Data

18298938

Source:http://linkedlifedata.com/resource/pubmed/id/18298938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-4-8
pubmed:abstractText
Oligonucleotide arrays are one of the most used technologies in genotyping and gene expression experiments. Here, a previously developed chitosan-based platform is characterized in its binding of unmodified oligonucleotides, increasing the cost effectiveness of the microarray production process. The unmodified oligonucleotides on the activated chitosan surface showed the same or better performances than amino-modified probes. Moreover, we show applications in genotyping (a ligation detection reaction experiment on cyanobacteria) and in gene expression (on peach RNA samples). The platform was demonstrated to be reliable, robust, and reproducible for immobilizing unmodified oligonucleotides in high quality oligonucleotide array fabrication.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1096-0309
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
376
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
39-43
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Anchoring of unmodified oligonucleotides on a chitosan-based array: applications to genotyping and gene expression.
pubmed:affiliation
Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche, LITA, via F.lli Cervi, 93 20090 Segrate (MI), Italy. clarissa.consolandi@itb.cnr.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't