Source:http://linkedlifedata.com/resource/pubmed/id/18294705
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-3-18
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| pubmed:abstractText |
Foot-and-mouth disease (FMD) is a highly contagious and economically devastating vesicular disease of cloven-hoofed animals. In this study, three amino acid residues 21-60, 141-160 and 200-213 from VP1 protein of FMDV were selected as multiple-epitopes (VPe), and a recombinant adenovirus expressing the multiple-epitopes fused with porcine interferon alpha (rAd-pIFN alpha-VPe) was constructed. Six groups of female BALB/c mice (18 mice per group) were inoculated subcutaneously (s.c.) twice at 2-week intervals with the recombinant adenoviruses and the immune responses were examined. Following this the protective efficacy of rAd-pIFN alpha-VPe was examined in guinea pigs and swine. The results showed that both FMDV-specific humoral and cell-mediated immune responses could be induced by rAd-VPe and increased when rAd-pIFN alpha is included in this regime in mice model. Moreover, the levels of the immune responses in the group inoculated with rAd-pIFN alpha-VPe were significantly higher than the group inoculated with rAd-VPe plus rAd-pIFN alpha. All guinea pigs and swine vaccinated with rAd-pIFN alpha-VPe were completely protected from viral challenge. It demonstrated that recombinant adenovirus rAd-pIFN alpha-VPe might be an attractive candidate vaccine for preventing FMDV infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/VP1 protein, Foot-and-mouth...,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0166-0934
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
144-52
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| pubmed:meshHeading |
pubmed-meshheading:18294705-Adenoviridae,
pubmed-meshheading:18294705-Animals,
pubmed-meshheading:18294705-Antibodies, Viral,
pubmed-meshheading:18294705-Capsid Proteins,
pubmed-meshheading:18294705-Cell Line,
pubmed-meshheading:18294705-Cytokines,
pubmed-meshheading:18294705-Epitopes,
pubmed-meshheading:18294705-Female,
pubmed-meshheading:18294705-Foot-and-Mouth Disease,
pubmed-meshheading:18294705-Foot-and-Mouth Disease Virus,
pubmed-meshheading:18294705-Guinea Pigs,
pubmed-meshheading:18294705-Humans,
pubmed-meshheading:18294705-Interferon-alpha,
pubmed-meshheading:18294705-Mice,
pubmed-meshheading:18294705-Recombinant Fusion Proteins,
pubmed-meshheading:18294705-Swine,
pubmed-meshheading:18294705-Swine Diseases,
pubmed-meshheading:18294705-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:18294705-Vaccines, Synthetic,
pubmed-meshheading:18294705-Viral Vaccines
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| pubmed:year |
2008
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| pubmed:articleTitle |
Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine.
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| pubmed:affiliation |
Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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