| pubmed-article:18292796 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18292796 | lifeskim:mentions | umls-concept:C0006826 | lld:lifeskim |
| pubmed-article:18292796 | lifeskim:mentions | umls-concept:C0003692 | lld:lifeskim |
| pubmed-article:18292796 | lifeskim:mentions | umls-concept:C0851346 | lld:lifeskim |
| pubmed-article:18292796 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:18292796 | lifeskim:mentions | umls-concept:C0162388 | lld:lifeskim |
| pubmed-article:18292796 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:18292796 | pubmed:dateCreated | 2008-4-9 | lld:pubmed |
| pubmed-article:18292796 | pubmed:abstractText | We previously demonstrated that 15-LOX-2 is significantly reduced in head and neck carcinoma and restoration of 15-LOX-2 expression results in tumor inhibition in HNC. The aim of this study is to evaluate 15-LOX-2 as a candidate for targeted radiotherapy. Molecular subcloning was performed to create a radiation-inducible 15-LOX-2 expression vector in which the full-length 15-LOX-2 cDNA was inserted downstream the recombinant Egr-1 promoter. The radiation-induced downregulations of 15-LOX-2 protein (twofold up) and its main metabolite 15S-HETE (threefold up) were observed in HNC cells transfected with the 15-LOX-2 expression vector after 4 Gy of radiation. Radiation-induced upregulation of 15-LOX-2 resulted in significant induction of apoptosis in HNC cells. Furthermore, survival colony formation was significantly reduced by 4 Gy in the HNC cells containing the 15-LOX-2 expression vector compared with the controls. Radiation-induced upregulation of 15-LOX-2 results in significant induction of apoptosis and enhances killing effect of radiotherapy in HNC. In addition, exogenous addition of 15S-HETE at high concentrations (>/=10 muM) but not at low concentrations induced upregulation of its endogenous ligand PPARgamma. In conclusion, synergistic effect between radiation and 15-LOX-2 was observed in killing HNC. 15-LOX-2 may be a potential target in radiation-targeted therapy of HNC. The 15-LOX-2 inhibition may not be PPARgamma dependent. | lld:pubmed |
| pubmed-article:18292796 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18292796 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18292796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18292796 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18292796 | pubmed:month | May | lld:pubmed |
| pubmed-article:18292796 | pubmed:issn | 1476-5500 | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:WangDD | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:FengYY | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:YanoYY | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:WuHH | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:LiX AXA | lld:pubmed |
| pubmed-article:18292796 | pubmed:author | pubmed-author:SchultzC JCJ | lld:pubmed |
| pubmed-article:18292796 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18292796 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:18292796 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18292796 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18292796 | pubmed:pagination | 323-30 | lld:pubmed |
| pubmed-article:18292796 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:18292796 | pubmed:meshHeading | pubmed-meshheading:18292796... | lld:pubmed |
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| pubmed-article:18292796 | pubmed:meshHeading | pubmed-meshheading:18292796... | lld:pubmed |
| pubmed-article:18292796 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18292796 | pubmed:articleTitle | Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer. | lld:pubmed |
| pubmed-article:18292796 | pubmed:affiliation | Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA. | lld:pubmed |
| pubmed-article:18292796 | pubmed:publicationType | Journal Article | lld:pubmed |
