Source:http://linkedlifedata.com/resource/pubmed/id/18292796
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2008-4-9
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pubmed:abstractText |
We previously demonstrated that 15-LOX-2 is significantly reduced in head and neck carcinoma and restoration of 15-LOX-2 expression results in tumor inhibition in HNC. The aim of this study is to evaluate 15-LOX-2 as a candidate for targeted radiotherapy. Molecular subcloning was performed to create a radiation-inducible 15-LOX-2 expression vector in which the full-length 15-LOX-2 cDNA was inserted downstream the recombinant Egr-1 promoter. The radiation-induced downregulations of 15-LOX-2 protein (twofold up) and its main metabolite 15S-HETE (threefold up) were observed in HNC cells transfected with the 15-LOX-2 expression vector after 4 Gy of radiation. Radiation-induced upregulation of 15-LOX-2 resulted in significant induction of apoptosis in HNC cells. Furthermore, survival colony formation was significantly reduced by 4 Gy in the HNC cells containing the 15-LOX-2 expression vector compared with the controls. Radiation-induced upregulation of 15-LOX-2 results in significant induction of apoptosis and enhances killing effect of radiotherapy in HNC. In addition, exogenous addition of 15S-HETE at high concentrations (>/=10 muM) but not at low concentrations induced upregulation of its endogenous ligand PPARgamma. In conclusion, synergistic effect between radiation and 15-LOX-2 was observed in killing HNC. 15-LOX-2 may be a potential target in radiation-targeted therapy of HNC. The 15-LOX-2 inhibition may not be PPARgamma dependent.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 15-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/EGFR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1476-5500
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
323-30
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:18292796-Apoptosis,
pubmed-meshheading:18292796-Arachidonate 15-Lipoxygenase,
pubmed-meshheading:18292796-Cell Line, Tumor,
pubmed-meshheading:18292796-Cell Survival,
pubmed-meshheading:18292796-Combined Modality Therapy,
pubmed-meshheading:18292796-Genes, Reporter,
pubmed-meshheading:18292796-Genetic Vectors,
pubmed-meshheading:18292796-Head and Neck Neoplasms,
pubmed-meshheading:18292796-Humans,
pubmed-meshheading:18292796-Lipoxygenase,
pubmed-meshheading:18292796-Luciferases,
pubmed-meshheading:18292796-Promoter Regions, Genetic,
pubmed-meshheading:18292796-Receptor, Epidermal Growth Factor
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pubmed:year |
2008
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pubmed:articleTitle |
Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
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pubmed:affiliation |
Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, WI, USA.
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pubmed:publicationType |
Journal Article
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