Source:http://linkedlifedata.com/resource/pubmed/id/18292277
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2008-2-22
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pubmed:abstractText |
A PCR-single strand conformation polymorphism protocol has been developed for rapid genotyping of the yak kappa-casein gene. A total of 307 yaks from the Tianzhu White, Jiulong, Maiwa, and Datong breeds in China were genotyped at the kappa-casein locus using the protocol developed in the present study. A polymorphism of kappa-casein gene exon 4 has been identified in Tianzhu White breed by evaluating genomic DNA. The polymorphic site consists of a single nucleotide substitution G-->C at position 362 of the exon 4, resulting in an AA substitution from Arg to Pro at position 121 of the AA sequence and in 2 alleles named, respectively, G and C based on nucleotide 362. The occurrence of allele C in the Tianzhu White breed was high with an allele frequency of 0.15. However, allele C appears to be absent in the yaks from Jiulong, Maiwa, and Datong breeds.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1525-3198
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1204-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:18292277-Amino Acid Sequence,
pubmed-meshheading:18292277-Animals,
pubmed-meshheading:18292277-Base Sequence,
pubmed-meshheading:18292277-Caseins,
pubmed-meshheading:18292277-Cattle,
pubmed-meshheading:18292277-China,
pubmed-meshheading:18292277-DNA,
pubmed-meshheading:18292277-Female,
pubmed-meshheading:18292277-Gene Frequency,
pubmed-meshheading:18292277-Genetic Variation,
pubmed-meshheading:18292277-Genotype,
pubmed-meshheading:18292277-Molecular Sequence Data,
pubmed-meshheading:18292277-Polymerase Chain Reaction,
pubmed-meshheading:18292277-Polymorphism, Genetic,
pubmed-meshheading:18292277-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:18292277-Species Specificity
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pubmed:year |
2008
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pubmed:articleTitle |
Short communication: characterization of a kappa-casein genetic variant in the Chinese yak, Bos grunniens.
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pubmed:affiliation |
Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang 110161, China. wlb7411@163.com <wlb7411@163.com>
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pubmed:publicationType |
Journal Article
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