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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1991-7-30
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| pubmed:abstractText |
Screening of an induced Ehrlich ascites cell-derived lambda gt11 cDNA library with an antibody (GP1), immunoreacting specifically with centrosomal antigen(s) of interphase and mitotic cells of different species, released a partial cDNA clone (lambda P10A) encoding the carboxy-terminal section of a centrosome-specific antigen. This specificity of the clone lambda P10A could be verified by lacZ-directed antigen expression from Escherichia coli Y1089 lysogenized with the recombinant phage lambda P10A and subsequent production of centrosome-specific antibodies by means of the recombinant antigen. Using the lambda P10A insert as a probe, two types of cDNA clones were identified in a lambda gt10 cDNA library by plaque-hybridization. The inserts of PN1 type clones were 1.2 kb (kilobases) and those of PN5 type clones were 2.2 kb in length. The DNA sequence of a PN1 type clone revealed its full-length cDNA nature. The open reading frame of PN1 encodes a rather hydrophilic and highly charged 34.5 x 10(3) Mr polypeptide comprising short but apparently significant strings of 100% sequence identity with the major nuclear lamina polypeptides lamins A/C and lamin B. Restriction enzyme mapping of PN1 and PN5 inserts, cross-hybridization experiments and comparison of overlapping DNA sequences indicate that the 1.2 kb and 2.2 kb cDNAs code for the same 34.5 x 10(3) Mr polypeptide, termed centrosomin A. Western blots of Ehrlich ascites cell proteins show a second, larger GP1 antigen (centrosomin B) whose cDNA has not been cloned. It remains to be investigated whether centrosomin B is encoded by a second mRNA or whether it reflects an oligomeric or a postranslationally modified form of centrosomin A.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0021-9533
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
98 ( Pt 1)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
37-43
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:1829085-Amino Acid Sequence,
pubmed-meshheading:1829085-Animals,
pubmed-meshheading:1829085-Antibodies, Monoclonal,
pubmed-meshheading:1829085-Antigens,
pubmed-meshheading:1829085-Antigens, Nuclear,
pubmed-meshheading:1829085-Bacteriophage lambda,
pubmed-meshheading:1829085-Base Sequence,
pubmed-meshheading:1829085-Cloning, Molecular,
pubmed-meshheading:1829085-DNA,
pubmed-meshheading:1829085-DNA, Recombinant,
pubmed-meshheading:1829085-DNA Probes,
pubmed-meshheading:1829085-Gene Library,
pubmed-meshheading:1829085-Genetic Code,
pubmed-meshheading:1829085-Mice,
pubmed-meshheading:1829085-Molecular Probes,
pubmed-meshheading:1829085-Molecular Sequence Data,
pubmed-meshheading:1829085-Organelles,
pubmed-meshheading:1829085-Recombinant Fusion Proteins
|
| pubmed:year |
1991
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| pubmed:articleTitle |
Murine cDNAs coding for the centrosomal antigen centrosomin A.
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| pubmed:affiliation |
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, FRG.
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| pubmed:publicationType |
Journal Article
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