Linked Life Data

18290602

Source:http://linkedlifedata.com/resource/pubmed/id/18290602

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-3-20
pubmed:abstractText
We have covalently attached multiple photoluminescent silicon nanoparticles (SNs) to streptavidin molecules. Conjugation of SNs to a target protein is achieved using the multistage photoassisted procedure. In a first step, the terminal hydrogen in the freshly prepared SNs is substituted with an alkane monolayer that serves as a platform for chemical linkage to a heterobifunctional cross-linker: 4-azido-2,3,5,6-tetrafluorobenzoic acid, succinimidyl ester. A resulting surface coating stabilizes nanoparticles against oxidation and aggregation. Next, an open end of bifunctional cross-linker-diazirine succinimidyl ester is reacted with carboxyl moieties of streptavidin and forms an amide bond. Gel and capillary electrophoresis of the SN-streptavidin complex demonstrated separate elution of the conjugation product and unreacted protein. Then, the number of SNs per protein molecule was determined by measuring complex charge variation by capillary electrophoresis. Conjugate functionality was tested by allowing it to interact with biotinylated polystyrene microbeads. Intense photoluminescence at carefully washed microbeads demonstrated selective binding of silicon nanoparticle bearing streptavidin to biotinylated microbeads. The high quantum yield of streptavidin-SN conjugate in combination with the small size and biocompatibility of silicon nanoparticles presents an attractive platform for the fluorescence labeling in diverse bioassays.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1043-1802
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
680-5
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Conjugation of the photoluminescent silicon nanoparticles to streptavidin.
pubmed:affiliation
Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA.
pubmed:publicationType
Journal Article