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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1991-7-22
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pubmed:abstractText |
We show using mild extraction procedures that the p53 proto-oncogene forms a complex with adenovirus 5 E1b-58 kD during infection. These complexes are detected as coimmunoprecipitates from radiolabeled extracts of adenovirus infected cells on SDS-PAGE. Furthermore, adenovirus mutants with defects in E1b-58 kD fail to form complexes, whereas mutants in other early region genes still show evidence of complex. Using a panel of monoclonal antibodies to mouse p53, we show that antibodies reacting with N-terminal epitopes on p53, displace E1b-58 kD. This result suggests that E1b-58 kD binds to an N-terminal region of mouse p53. In addition, in a transient transfection assay in monkey COS cells, we show that an N-terminal deletion mutant of mouse p53 does not bind to E1b-58 kD but wild-type mouse p53 does bind. This result again suggests that E1b-58 kD binds an N-terminal determinant on p53.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0950-9232
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
781-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1828873-Adenoviridae,
pubmed-meshheading:1828873-Adenovirus Early Proteins,
pubmed-meshheading:1828873-Animals,
pubmed-meshheading:1828873-Antibodies, Monoclonal,
pubmed-meshheading:1828873-Binding Sites,
pubmed-meshheading:1828873-Cell Line,
pubmed-meshheading:1828873-Chromosome Deletion,
pubmed-meshheading:1828873-HeLa Cells,
pubmed-meshheading:1828873-Humans,
pubmed-meshheading:1828873-Molecular Weight,
pubmed-meshheading:1828873-Oncogene Proteins, Viral,
pubmed-meshheading:1828873-Protein Binding,
pubmed-meshheading:1828873-Transfection,
pubmed-meshheading:1828873-Tumor Suppressor Protein p53
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pubmed:year |
1991
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pubmed:articleTitle |
Adenovirus E1b-58 kD antigen binds to p53 during infection of rodent cells: evidence for an N-terminal binding site on p53.
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pubmed:affiliation |
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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