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rdf:type |
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lifeskim:mentions |
umls-concept:C0037083,
umls-concept:C0079284,
umls-concept:C0205266,
umls-concept:C0230653,
umls-concept:C0449560,
umls-concept:C0597357,
umls-concept:C0871261,
umls-concept:C1167250,
umls-concept:C1546857,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1710082,
umls-concept:C2911692
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pubmed:issue |
2
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pubmed:dateCreated |
2008-5-29
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pubmed:abstractText |
We have investigated the role of NAADP-mediated Ca(2+) mobilization in endothelin (ET) signaling via endothelin receptor subtype A (ETA) and endothelin receptor subtype B (ETB) in rat peritubular smooth muscle cells. Microinjection and extracellular application of NAADP were both able to elicit Ca(2+) release which was blocked by inhibitory concentrations of NAADP, by impairing Ca(2+) uptake in acidic stores with bafilomycin, and by thapsigargin. Ca(2+) release in response to selective ETB stimulation was abolished by inhibition of NAADP signaling through the same strategies, while these treatments only partially impaired ETA-dependent Ca(2+) signaling, showing that transduction of the ETB signal is dependent on NAADP. In addition, we show that lipid rafts/caveolae contain ETA, ETB, and NAADP/cADPR generating enzyme CD38 and that stimulation of ETB receptors results in increased CD38 activity; interestingly, ETB- (but not ETA-) mediated Ca(2+) responses were antagonized by disruption of lipid rafts/caveolae with methyl-beta-cyclodextrin. These data demonstrate a primary role of NAADP in ETB-mediated Ca(2+) signaling and strongly suggest a novel role of lipid rafts/caveolae in triggering ET-induced NAADP signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cd38 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/IRL 1620,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NAADP,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin B,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Cyclodextrins,
http://linkedlifedata.com/resource/pubmed/chemical/methyl-beta-cyclodextrin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1097-4652
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
216
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
396-404
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pubmed:meshHeading |
pubmed-meshheading:18288637-Animals,
pubmed-meshheading:18288637-Antigens, CD38,
pubmed-meshheading:18288637-Calcium,
pubmed-meshheading:18288637-Calcium Signaling,
pubmed-meshheading:18288637-Caveolae,
pubmed-meshheading:18288637-Caveolin 1,
pubmed-meshheading:18288637-Cells, Cultured,
pubmed-meshheading:18288637-Endothelin-1,
pubmed-meshheading:18288637-Endothelins,
pubmed-meshheading:18288637-Enzyme Inhibitors,
pubmed-meshheading:18288637-Male,
pubmed-meshheading:18288637-Membrane Glycoproteins,
pubmed-meshheading:18288637-Membrane Microdomains,
pubmed-meshheading:18288637-Myocytes, Smooth Muscle,
pubmed-meshheading:18288637-NADP,
pubmed-meshheading:18288637-Peptide Fragments,
pubmed-meshheading:18288637-Rats,
pubmed-meshheading:18288637-Rats, Wistar,
pubmed-meshheading:18288637-Receptor, Endothelin A,
pubmed-meshheading:18288637-Receptor, Endothelin B,
pubmed-meshheading:18288637-Seminiferous Tubules,
pubmed-meshheading:18288637-Signal Transduction,
pubmed-meshheading:18288637-beta-Cyclodextrins
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pubmed:year |
2008
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pubmed:articleTitle |
NAADP-induced Ca(2+ signaling in response to endothelin is via the receptor subtype B and requires the integrity of lipid rafts/caveolae.
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pubmed:affiliation |
Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Histology and Medical Embryology, University of Rome La Sapienza, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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