Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2008-4-14
pubmed:abstractText
Theiler's murine encephalomyelitis virus (TMEV), a member of the Cardiovirus genus in the family Picornaviridae, is a highly cytolytic virus that produces necrotic death in rodent cells except for macrophages, which undergo apoptosis. In the present study we have analyzed the kinetics of BeAn virus infection in M1-D cells, in order to temporally relate virus replication to the apoptotic signaling events. Apoptosis was associated with early exponential virus growth from 1 to 12 h postinfection (p.i.); however, >/=80% of peak infectivity was lost by 16 to 24 h p.i. The pan-caspase inhibitor qVD-OPh led to significantly higher virus yields, while zVAD-fmk completely inhibited virus replication until 10 h p.i., precluding its assessment in apoptosis. In contrast, while zVAD-fmk significantly inhibited BeAn virus replication in BHK-21 cells at 12 and 16 h p.i., virus replication at these time points was not altered by qVD-OPh. Bax translocation into mitochondria, efflux of cytochrome c into the cytoplasm, and activation of caspases 9 and 3 between approximately 8 and 12 h p.i. (all hallmarks of the intrinsic apoptotic pathway) were transiently inhibited by expression of Bcl-2, which is not expressed in M1-D cells. Thus, BeAn virus infection in M1-D macrophages, which restricts virus replication, provides a potential mechanism for modulating TMEV neurovirulence during persistence in the mouse central nervous system.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-10074176, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-10085063, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-10423138, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-10617566, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-11099404, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-11390594, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-11457886, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-11559814, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-12438607, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-12815277, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-14987997, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-15031729, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-15246649, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-15542687, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-16015365, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-164412, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-16497721, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-16690857, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-17062731, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-17289999, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-17454448, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-2784140, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-2849023, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-4561027, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-6192587, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-7682708, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-7778279, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-7884902, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-8468358, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-8794327, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-8985406, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9002967, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9060706, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9382873, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9670005, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9671497, http://linkedlifedata.com/resource/pubmed/commentcorrection/18287228-9721092
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1098-5514
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4502-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Theiler's virus-induced intrinsic apoptosis in M1-D macrophages is Bax mediated and restricts virus infectivity: a mechanism for persistence of a cytolytic virus.
pubmed:affiliation
Department of Microbiology-Immunology, MC 790, University of Illinois at Chicago, 835 South Wolcott, Chicago, IL 60612-7344, USA. hlipton@uic.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural