Linked Life Data

1828714

Source:http://linkedlifedata.com/resource/pubmed/id/1828714

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-7-22
pubmed:abstractText
Structural, biochemical, and genetic evidence has demonstrated there are three inner dynein arm subforms, I1, I2, and I3, which differ in organization and composition (see Piperno et al.: J. Cell Biol. 110:379-389, 1990). Using dynein extracted from Chlamydomonas outer dynein armless mutant pf28, we have begun to define the structural and functional properties of isolated inner arm subforms. Inner dynein arm I1 was purified either by sucrose density gradient centrifugation or microtubule binding affinity. I1, composed of heavy chains 1 alpha and 1 beta, sedimented at 21S and selectively bound to and cross-linked purified microtubules in an ATP-sensitive manner. Deep etch electron microscopy revealed that the 21S sedimenting fraction contained two-headed structures in which large globular heads are connected by long, flexible-stem domains. In contrast, components derived from I2 and I3 sedimented as a mixture of 11S particles with single globular heads which did not bind to purified microtubules. Both the 21S and 11S sedimenting fractions supported microtubule translocation in in vitro motility assays. In 1 mM MgATP the I1-containing fraction produced very slow microtubule-gliding velocities (0.76 microns/sec) compared to the I2,I3-containing fraction (4.1 microns/sec).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0886-1544
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-68
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Microtubule binding and translocation by inner dynein arm subtype I1.
pubmed:affiliation
Department of Anatomy and Cell Biology, Emory University, School of Medicine, Atlanta 30322.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.