Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-4-25
pubmed:abstractText
Sordarin and its derivatives are antifungal compounds of potential clinical interest. Despite the highly conserved nature of the fungal and mammalian protein synthesis machineries, sordarin is a selective inhibitor of protein synthesis in fungal organisms. In cells sensitive to sordarin, its mode of action is through preventing the release of translation elongation factor 2 (eEF2) during the translocation step, thus blocking protein synthesis. To further investigate the cellular components required for the effects of sordarin in fungal cells, we have used the haploid deletion collection of Saccharomyces cerevisiae to systematically identify genes whose deletion confers sensitivity or resistance to the compound. Our results indicate that genes in a number of cellular pathways previously unknown to play a role in sordarin response are involved in its growth effects on fungal cells and reveal a specific requirement for the diphthamidation pathway of cells in causing eEF2 to be sensitive to the effects of sordarin on protein synthesis. Our results underscore the importance of the powerful genomic tools developed in yeast (Saccharomyces cerevisiae) to more comprehensively understanding the cellular mechanisms involved in the response to therapeutic agents.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-10400702, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-10436161, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-10802651, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-10835368, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-11083645, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-11158355, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-11849550, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-11994165, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12073338, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12134146, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12140549, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12431279, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12745995, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12859903, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-12940988, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-14976550, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-1508200, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-15273103, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-15316019, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-15485916, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-15769872, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-15860374, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-16107839, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-1631110, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-16390459, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-16897709, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-16929303, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-16950777, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-1706664, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-17446867, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-17981122, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-5485912, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-7000782, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-7651393, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-7651424, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-8406038, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9452424, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9531986, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9736548, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9736549, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9737960, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9756779, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9797217, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9822666, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9843429, http://linkedlifedata.com/resource/pubmed/commentcorrection/18285480-9988728
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1098-6596
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1623-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A chemical genomic screen in Saccharomyces cerevisiae reveals a role for diphthamidation of translation elongation factor 2 in inhibition of protein synthesis by sordarin.
pubmed:affiliation
Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, CSIC and Universidad de Salamanca, Salamanca, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't