Source:http://linkedlifedata.com/resource/pubmed/id/18276611
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2008-2-26
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pubmed:abstractText |
Transthyretin (TTR)-containing amyloid fibrils are deposited in cardiac tissue as a natural consequence of aging. A large number of inherited mutations lead to amyloid diseases by accelerating TTR deposition in other organs. Amyloid formation is preceded by a disruption of the quaternary structure of TTR and conformational changes in the monomer. To study conformational changes preceding the formation of amyloid, we performed molecular dynamics simulations of the wild-type monomer, amyloidogenic variants (V30M, L55P, V122I) and a protective variant (T119M) at neutral and low pH. At low pH, the D strand dissociated from the beta-sheet to expose the A strand, consistent with experimental studies. In amyloidogenic variants and in the wild-type at low pH, there was a conformational change in the beta-sheets into alpha-sheet via peptide bond flips that was not observed at neutral pH in the wild-type monomer. The same residues participated in conversion in each amyloidogenic variant simulation, originating in the G strand between residues 106 and 109, with accelerated conversion at low pH. The T119M protective variant changed the local conformation of the H strand and suppressed the conversion observed in amyloidogenic variants.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1741-0126
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-95
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pubmed:meshHeading |
pubmed-meshheading:18276611-Amino Acid Sequence,
pubmed-meshheading:18276611-Amyloid Neuropathies,
pubmed-meshheading:18276611-Humans,
pubmed-meshheading:18276611-Hydrogen Bonding,
pubmed-meshheading:18276611-Hydrogen-Ion Concentration,
pubmed-meshheading:18276611-Models, Molecular,
pubmed-meshheading:18276611-Molecular Sequence Data,
pubmed-meshheading:18276611-Mutation,
pubmed-meshheading:18276611-Prealbumin,
pubmed-meshheading:18276611-Protein Conformation,
pubmed-meshheading:18276611-Protein Folding
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pubmed:year |
2008
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pubmed:articleTitle |
Different disease-causing mutations in transthyretin trigger the same conformational conversion.
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pubmed:affiliation |
Department of Bioengineering, University of Washington, Seattle, WA 98195-5061, USA.
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pubmed:publicationType |
Journal Article
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