Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-6-6
|
| pubmed:databankReference | |
| pubmed:abstractText |
UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-T I; EC 2.4.1.101) is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. We have isolated two overlapping genomic DNA clones which span 18 kilobases (kb) containing a single 2.5 kb exon for GlcNAc-T I. The exon includes most of the 5'-untranslated region, the complete coding sequence (1335 bases) for GlcNAc-T I (445 amino acids) and the complete 3'-untranslated region. The remaining exon (or exons) is at least 2.0 kb upstream of the intron-exon junction. Transient transfection of either clone into Lec 1 Chinese hamster ovary cell mutants (which lack GlcNAc-T I) indicates the presence of a promoter responsible for expression of a truncated transcript. Southern blot analysis indicates that the gene exists in single copy in the human genome and is located on chromosome 5. The human and rabbit enzymes are 85% similar at the nucleotide sequence level and 92% similar at the amino acid sequence level.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
176
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
608-15
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1827260-Amino Acid Sequence,
pubmed-meshheading:1827260-Animals,
pubmed-meshheading:1827260-Base Sequence,
pubmed-meshheading:1827260-Blotting, Northern,
pubmed-meshheading:1827260-Chromosomes, Human, Pair 5,
pubmed-meshheading:1827260-Cloning, Molecular,
pubmed-meshheading:1827260-Cricetinae,
pubmed-meshheading:1827260-Cricetulus,
pubmed-meshheading:1827260-Exons,
pubmed-meshheading:1827260-Gene Expression,
pubmed-meshheading:1827260-Glucosyltransferases,
pubmed-meshheading:1827260-Humans,
pubmed-meshheading:1827260-Introns,
pubmed-meshheading:1827260-Liver,
pubmed-meshheading:1827260-Models, Molecular,
pubmed-meshheading:1827260-Molecular Sequence Data,
pubmed-meshheading:1827260-N-Acetylglucosaminyltransferases,
pubmed-meshheading:1827260-Rabbits,
pubmed-meshheading:1827260-Restriction Mapping,
pubmed-meshheading:1827260-Transfection
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene.
|
| pubmed:affiliation |
Research Institute, Hospital for Sick Children, Toronto, Canada.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|