Linked Life Data

18272397

Source:http://linkedlifedata.com/resource/pubmed/id/18272397

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-3-10
pubmed:databankReference
pubmed:abstractText
Partial cDNA sequences of TCRgamma and CD3gamma/delta were isolated from the thymus of common carp (Cyprinus carpio L.) by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp TCRgamma and CD3gamma/delta were obtained by means of 3' RACE and 5' RACE, respectively. The full length of carp TCRgamma chain is 1368bp and encodes 326 amino acids including a signal peptide region of 19 amino acids and a transmembrane region of 23 amino acids at the C-terminal region from aa 291 to 313. The V region of carp TCRgamma contains 109 amino acids, the core motif FGXG in J segment was also found in carp TCRgamma. The C region of carp TCRgamma contains the characteristic CX6PX6WX45C motif. The CP region of carp TCR Cgamma contains 37 amino acids. The full length of carp CD3gamma/delta is 790bp and encodes 175 amino acids including a signal peptide region of 17 amino acids and a transmembrane region of 23 amino acids from aa 93 to 115. Similar to other known CD3gamma/deltas, four cysteine residues in the extracellular domain and an immunoreceptor tyrosine-based activation motif ITAM (YxxL/Ix6-8YxxL/I) in the intracellular domain are also included in carp CD3gamma/delta. Differing from other known CD3gamma/deltas, carp CD3gamma/delta lacks the CXXCXE motif in the extracellular domain. RT-PCR analysis demonstrated that the expression of TCRgamma gene was mainly in the thymus and gill of 6-month carp, but in 18-month carp, TCRgamma gene was detected in all the examined tissues. The expression of CD3gamma/delta gene was detected in all examined tissues of 6 and 18-month carp; among them, the highest expression level was in the thymus of 6-month carp. In situ hybridization showed that CD3gamma/delta-expressing cells were widely distributed in the head kidney, spleen and kidney of carp, whereas in the thymus, they were densely distributed in the lymphoid outer zone and scattered in the epithelioid inner zone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1050-4648
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
412-25
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Molecular cloning and characterization of common carp (Cyprinus carpio L.) TCRgamma and CD3gamma/delta chains.
pubmed:affiliation
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't