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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0034790,
umls-concept:C0040648,
umls-concept:C0086222,
umls-concept:C0205245,
umls-concept:C0591833,
umls-concept:C0759310,
umls-concept:C1332711,
umls-concept:C1420642,
umls-concept:C1514562,
umls-concept:C1705733,
umls-concept:C2003941
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pubmed:issue |
5
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pubmed:dateCreated |
1991-6-4
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pubmed:abstractText |
CD3-epsilon gene expression is confined to the T cell lineage. We have recently identified and cloned a human transcription factor, TCF-1, that binds to a functional element in the T lymphocyte-specific enhancer of CD3-epsilon. In a panel of human cell lines, TCF-1 expression was restricted to T lineage cells. TCF-1 belonged to a novel family of genes that contain the so-called high mobility group 1 (HMG) box. Here we report the cloning of murine TCF-1. Two splice alternatives were identified that were not previously observed in human TCF-1. Murine and human TCF-1 displayed a 95.5% overall amino acid homology. Recombinant murine and human TCF-1 recognized the same sequence motif in the CD3-epsilon enhancer as judged by gel retardation and methylation interference assays. With the murine cDNA clones several aspects of TCF-1 were analyzed. First, deletion analysis revealed that a region of TCF-1 containing the HMG box was sufficient for sequence-specific binding. Second, by high stringency Northern blotting and in situ hybridization, TCF-1 expression was shown to be confined to the thymus and to the T cell areas of the spleen. Third, TCF-1 bound specifically to a functional T cell-specific element in the T cell receptor alpha (TCR-alpha) enhancer. The T lineage-specific expression and the affinity for functional motifs in the TCR-alpha and CD3-epsilon enhancers imply an important role for TCF-1 in the establishment of the mature T cell phenotype.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-1695712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2083253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2136828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2142304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2230652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2330041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2444404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2504497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2524381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2550138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2583122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2673023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2725678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2776214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2788889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2848550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2900761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2902927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2902928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2904653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-2904654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-3098850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-3267235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-3289580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-3296191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-3690668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1827138-6313477
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1133-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1827138-Amino Acid Sequence,
pubmed-meshheading:1827138-Animals,
pubmed-meshheading:1827138-Antigens, CD3,
pubmed-meshheading:1827138-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1827138-B-Lymphocytes,
pubmed-meshheading:1827138-Base Sequence,
pubmed-meshheading:1827138-Blotting, Northern,
pubmed-meshheading:1827138-Cell Line,
pubmed-meshheading:1827138-Chromosome Deletion,
pubmed-meshheading:1827138-DNA,
pubmed-meshheading:1827138-Enhancer Elements, Genetic,
pubmed-meshheading:1827138-Gene Expression,
pubmed-meshheading:1827138-Humans,
pubmed-meshheading:1827138-Mice,
pubmed-meshheading:1827138-Molecular Sequence Data,
pubmed-meshheading:1827138-Receptors, Antigen, T-Cell,
pubmed-meshheading:1827138-Recombination, Genetic,
pubmed-meshheading:1827138-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1827138-T-Lymphocytes,
pubmed-meshheading:1827138-Transcription Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Cloning of murine TCF-1, a T cell-specific transcription factor interacting with functional motifs in the CD3-epsilon and T cell receptor alpha enhancers.
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pubmed:affiliation |
Department of Clinical Immunology, University Hospital Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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